Mouse Models to Study MiRP3 Physiology

描述(由申请人提供)： 钾离子通道催化K+离子跨细胞膜移动，其功能对细胞电活动和电解质稳态是必不可少的。许多K+通道受辅助亚单位蛋白的调节，这是正常生理功能所必需的，并使通道能够适应环境条件的变化。本申请描述了水貂相关多肽超家族中的一个新成员--β亚基MiRP3的研究，它可以调节这种K+通道的特性。我提出的初步证据表明，MiRP3在调节心脏电生理、平滑肌功能和肾脏钾分泌方面发挥作用。在此，我提出了一个研究项目(K08DK068188)的扩展，该项目将利用动物模型来测试miRP3的这些假定的生理作用。我建议使用动物模型来评估MiRP3蛋白在心脏电生理、平滑肌功能和肾脏钾分泌过程中的生理学作用。

DESCRIPTION (provided by applicant): Potassium channels catalyze the movement of K+ ions across the cell membrane, and their function is essential for cellular electrical activity and electrolyte homeostasis. Many K+ channels are regulated by accessory-subunit proteins, which can be required for normal physiologic function and allow for the channels to adapt to changes of environmental conditions. This application describes the study of the beta subunit MiRP3, a novel member of the MinK-related peptide superfamily, which can modulate the properties of such K+ channels. I present preliminary evidence that suggests a role for MiRP3 in modulating cardiac electrophysiology, smooth-muscle function, and renal potassium secretion. Herein, I propose an extension of research project (K08 DK068188) that will utilize animal models to test these putative physiologic roles for MiRP3. I propose to use animal models to evaluate the physiologic role of the MiRP3 protein in the processes of cardiac electrophysiology, smooth-muscle function, and renal potassium secretion.