Towards the Physiologic Function of MiRP-3

描述（由申请人提供）：MCSD提案：朝向MiRP-3的生理功能。 研究：钾通道催化钾离子跨细胞膜的运动，其功能对于细胞电活动和电解质稳态是必不可少的。许多K+通道由辅助亚基蛋白调节，其可以是正常生理功能所需的，并允许通道适应环境条件的变化。本申请描述了对β亚基MiRP-3的研究，MiRP-3是MinK相关肽超家族的新成员，其可以调节这种K+通道的性质。我们目前的初步证据表明，MiRP-3在调节血管平滑肌功能以及肾脏钾分泌和酸碱平衡的作用。我们提出了一个研究MiRP-3和生理学相关合作伙伴的计划。将评估MiRP-3对K+通道的影响以及这种调节如何影响生理过程。候选人：丹尼尔博士。Levy是一名离子通道生理学家（宾夕法尼亚大学）。Sch. Med.），这促使他决定选择内科住院医师（宾夕法尼亚大学医院）和肾脏科专科培训（耶鲁大学）。他现在是芝加哥大学肾脏科的讲师。他的职业目标是经营一个基础研究实验室，研究离子通道功能的机械原理及其对人类疾病的影响。提交该提案是为了支持他在生物物理学，生物化学和分子遗传学学科的职业发展，使他能够成为一名独立的研究者和学术上可行的临床科学家。 环境：主要发起人Steve Goldstein博士是钾通道研究和离子通道病的主要研究者。前三个MinK相关肽是在他的实验室中鉴定和表达的，该实验室具有丰富的生物物理学，分子生物学和分子遗传学专业知识。芝加哥大学的医学系和神经生物学、药理学和生理学系包括国际公认的离子通道研究骨干。

DESCRIPTION (provided by applicant): MCSD Proposal: Towards the physiologic function of MiRP-3. The research: Potassium channels catalyze the movement of K+ ions across the cell membrane, and their function is essential for cellular electrical activity and electrolyte homeostasis. Many K+ channels are regulated by accessory-subunit proteins, which can be required for normal physiologic function and allow for the channels to adapt to changes of environmental conditions. This application describes the study of the beta subunit MiRP-3, a novel member of the MinK-related peptide superfamily, which can modulate the properties of such K+ channels. We present preliminary evidence that suggests a role for MiRP-3 in modulating vascular smooth-muscle function as well as renal potassium secretion and acid-base homeostasis. We propose a plan to study MiRP-3 and a physiologically relevant partner. MiRP-3 will be evaluated for its effects on K+ channels and how such modulation can influence physiologic processes. The candidate: Dr. Daniel I. Levy trained as an ion-channel physiologist (Univ. Penn. Sch. Med.), which motivated his decision to choose a residency in Internal Medicine (Hosp. Univ. Pennsylvania) and subspecialty training in Nephrology (Yale). He is now an Instructor in the Nephrology section at the University of Chicago. His career goal is to run a basic research laboratory investigating the mechanistic principles of ion channel function and their implications for human diseases. This proposal is submitted to support his career development in the disciplines of biophysics, biochemistry, and molecular genetics, to enable him to become an independent investigator and academically viable clinician-scientist. The environment: The primary sponsor, Dr. Steve Goldstein, is a leading investigator in potassium channel research and ion channelopathies. The first three MinK-related peptides were identified and expressed in his laboratory, which is rich in biophysical, molecular biology, and molecular genetics expertise. The Department of Medicine and the Department of Neurobiology, Pharmacology & Physiology at the University of Chicago include an internationally recognized cadre of ion channel researchers.