Research Histopathology

自1981年以来，一个集中的组织学实验室为MDACC的研究人员提供组织学服务。 历史学研究设施的目的是提供技术支持和咨询， 开发和应用适当的新技术，以支持不断变化的研究需求，并保持 一致性和高质量需要执行这些基于组织的技术。RHF流程已修复 和冷冻标本，包括细胞系和小鼠胚胎制备物，用于常规组织学分析， 免疫组织化学和原位杂交;特殊染色;组织移植; 无DNA酶和RNA酶程序;冷冻切片;组织微阵列块切片;手术刀 显微解剖和免疫组织化学染色。RHF可供MDACC的所有教职员工使用， 优先考虑有同行评审赠款的研究人员。RHF最近占地740平方英尺， 在北校区的病理学系翻新的实验室空间和370平方英尺的R.E. “鲍勃”史密斯研究大楼在南校区。提供的服务包括快速准备 高质量的标准组织切片;根据选定项目的需要应用特殊技术， 包括制备冷冻切片和组织切片用于组织化学、免疫组织化学和免疫组织化学， 原位杂交分析;与研究人员协商，以定制技术和方法， 特殊需要;以及转介器官部位专科病理学家就 组织病理学激光捕获显微切割服务将在2007年秋季增加。RHF利用 国家的最先进的设备，以完成其工作，因为在过去两年中，两个实验室已取代 他们的设备，以更好地为用户服务。RHF目前拥有一个工作人员，其中包括一个共享的 资源主任，一名联合主任，一名经理/首席技术员，2名高级组织学技术员，2名 组织学研究技术员和2名组织学实验室助理。在过去的五年里， 被代表18个不同CCSG项目的287名研究者使用，使用率增加了569%。 拥有同行评审资金的用户占服务利用率的78%，占用户总数的53%。 未来的计划包括继续改进组织学收费数据库系统， 设备更换周期，南校区设施的扩建，以及激光捕获的激活 显微切割服务。

Since 1981, a centralized histology laboratory has provided histologic services to investigators at MDACC. The aim of the Research Histopathology Facility (RHF) is to provide technical support and consultation, develop and apply appropriate new techniques to support evolving research needs, and maintain the consistency and high quality needed to perform these tissue-based techniques. The RHF processes fixed and frozen specimens including cell line and mouse embryo preparations for histologic analysis in routine, thin and thick sections for immunohistochemistry and in situ hybridization; special staining; tissue transfer; DNAse- and RNAse-free procedures; frozen sections; sectioning of tissue microarray blocks; scalpel microdissection; and immunohistochemical staining. The RHF is available to all faculty members at MDACC, with preference given to investigators with peer-reviewed grants. The RHF occupies 740 sq ft of recently renovated laboratory space in the Department of Pathology on the North Campus and 370 sq ft in the R.E. "Bob" Smith Research Building on the South Campus. Among the services provided are rapid preparation of high-quality standard histologic sections; application of special technologies as needed for selected projects, including preparation of frozen sections and tissue sections for histochemical, immunohistochemical, and in situ hybridization analysis; consultation with investigators to tailor technologies and methodologies to their special needs; and referral for consultation by organ-site subspecialty pathologists on interpretation of histopathology. Laser capture microdissection services will be added in the Fall of 2007. The RHF utilizes state-of-the-art equipment to complete its work, as during the past 2 years both laboratories have replaced their equipment to better serve their users. The RHF currently maintains a staff that includes a Shared Resource Director, a Co-Director, a Manager/Chief Technologist, 2 Senior Histology Technicians, 2 Research Histology Technicians, and 2 Histology Laboratory Assistants. During the past 5 years, the RHF was used by 287 investigators representing 18 different CCSG Programs, and use increased by 569%. Users with peer-reviewed funding accounted for 78% of service utilization and represented 53% of users. Future plans include continued improvement of the Histology Chargeback Database System, continuation of the equipment replacement cycle, expansion of the South Campus facility, and activation of laser capture microdissection services.