Shared Resources-Animal Genomics

动物基因组学共享资源（AGS）的目的是创造（并通过冷冻保存， 维持）用于人类疾病（包括癌症）的转基因和敲除模型。具体目标是： 1)提供转基因和靶向构建体设计方面的咨询; 2）将DNA导入合子或 胚胎干细胞; 3）创建创始动物或嵌合体，其掺入所需的DNA添加物，或 4）冷冻保存小鼠胚胎，用于具有成本效益的长期储存基因工程 小鼠转基因小鼠服务创建于1989年。创造基因敲除小鼠的能力被添加到 1997.自2000年以来，已经进行了小鼠胚胎的冷冻保存。小说鼠标的创作 菌株，无论是通过转基因或同源重组，使许多成员的耶鲁癌， 中心（YCC），以确定基因的正常功能，以及如何干扰该功能可能导致 异常发育或疾病，如癌症。研究人员可以使用AGS来创建 突变参与人类疾病，从而提供了模型，可以研究的方式，人类 主体不能。创造这种诱导突变体的能力使研究人员能够有效地利用小鼠 遗传学解剖和分析复杂的发育途径，生化过程， 生物分子在哺乳动物中的相互作用达到了在这种技术存在之前无法达到的水平。 这使研究人员能够在比体外方法更深的层次上研究生物过程， 因为生物化学或细胞培养将允许，并且通过由以下引起的新表型的出现， 诱导突变，发现这些过程和途径之间的新关系。冷冻保存 小鼠品系使研究人员能够更有效地利用他们在研究中的财政资源， 保持有价值的菌株以备将来使用。YCC AGS每年生产40-60个转基因小鼠品系， 每年产生15-20个新的靶向突变小鼠模型。 动物基因组学服务自1989年以来一直是YCC的共享资源。AGS移动到新的空间 2002年，Anylan中心大楼落成，专为他们设计。 2005年，28名YCC研究人员使用了AGS，总计约1000人。62%的资源使用量。所有 2005年期间，YCC的使用来自8个YCC研究项目中的7个的同行评审科学家

The purpose of the Animal Genomics Shared Resource (AGS) is to create (and through cryopreservation, maintain) transgenic and knockout models for human diseases including cancer. The specific objectives are: 1) Provide consultation on transgene and targeting construct design; 2) Introduce DNA into zygotes or embryonic stem cells; 3) Create founder animals or chimeras that incorporate the desired, DNA addition or change; and 4) Cryopreserve mouse embryos for cost-effective, long-term storage of genetically engineered mice. The Transgenic mouse service was created in 1989. The ability to create knockout mice was added in 1997. Cryopreservation of mouse embryos has been performed since 2000. The creation of novel mouse strains, either by transgenesis or homologous recombination, allows many members of the Yale Cancer Center (YCC), to determine the normal function of genes and how perturbation of that function can result in aberrant development or diseases such as cancer. Investigators can use AGS to create direct analogs of mutations involved in human disease, thus providing models that can be studied in a way that human subjects cannot. The ability to create such induced mutants allows investigators to effectively use mouse genetics to dissect and analyze complicated developmental pathways, biochemical processes, and biomolecular interactions in a mammal to a level previously unattainable before such technology existed. This enables researchers to investigate biological processes at a far deeper level than in vitro methods such as biochemistry or cell culture will allow, and through the appearance of novel phenotypes resulting from induced mutations, discover new relationships between these processes and pathways. Cryopreservation of mouse strains allows investigators to more efficiently utilize their financial resources in research while maintaining valuable strains for future use. The YCC AGS produces 40-60 transgenic mouse strains/year, and creates 15-20 new targeted mutant mouse models/year. Animal Genomics Services has been a shared resource of the YCC since 1989. AGS moved into new space designed specifically for their use with the completion of The Anylan Center building in 2002. Twenty-eight YCC investigators used AGS in 2005 for a total of approx. 62% of all usage of the resource. All YCC usage during 2005 was from peer-reviewed scientists from 7 of 8 YCC Research Programs