High Throughput Screening

高通量筛查(NTS)是一种快速评估数十万 为了加速医学研究而影响生物系统的化学化合物。我们 提出HTS核心，其目标是使Gl孢子调查人员能够发现化学工具 促进对结直肠癌生理学的更好理解，并有可能帮助揭示新的靶点和 治疗机会。使用最先进的高温超导技术，并与Gl孢子调查人员合作， 范德比尔特化学生物学研究所的HTS设施已经开发并验证了HTS兼容性 旨在发现与以下方面相关的化学工具的检测：1)人类结肠癌中E-钙粘附素的调节 2)轴蛋白和(3-连环素)在新的体外检测系统中的稳定性；以及3)p120‘S在 调节E-钙粘附素活性并促进Colo205细胞系连接的形成。我们提供数据 展示我们执行HTS分析的能力，发现影响系统的化合物 以上，我们详细说明了我们计划使用HTS的产品来推进Gl孢子的目标。

High-throughput screening (NTS) is a means of rapidly assessing the ability of hundreds of thousands of chemical compounds to affect biological systems for the purpose of accelerating medical research. We propose an HTS core the goal of which is to enable Gl SPORE investigators to discover chemical tools to promote better understanding of colorectal cancer physiology and potentially to help reveal novel targets and therapeutic opportunities. Using state-of-the-art HTS technologies and working with Gl SPORE investigators, the Vanderbilt Institute of Chemical Biology's HTS facility has developed and validated HTS-compatible assays aimed at discovering chemical tools relevant to: 1) E-cadherin regulation in the human colon cancer cell line, SW620; 2) axin and (3-catenin stability in a novel in vitro assay system; and 3) p120's role in modulating E-cadherin activity and adherens junction formation in the Colo205 cell line. We present data demonstrating our ability to perform the HTS assays, discover compounds that affect the systems noted above, and we detail our plans to use the products of the HTS to further the aims of the Gl SPORE.