High Throughput Screening/Chemical Synthesis

高通量筛选（HTS）和化学合成（CS）共享资源提供了最新的 范德比尔特-英格拉姆癌症中心（VICC）研究人员利用HTS/CS的能力 以支持靶点发现、途径作图、化学工具发现和探索性临床前药物 发现研究HTS/CS包括一个包含超过170，0000个小分子的化学库，先进的液体 处理和广泛的信号检测模式，使研究人员能够解决问题 从纯化的无细胞系统中的蛋白质-蛋白质相互作用到菌落形成和形态学 使用自动化成像进行分析。该设施有能力解决所有常用的光学问题 技术（荧光，发光，动力学成像，高含量成像，荧光邻近， 折射率光谱（康宁史诗），自动电生理学，并在不久的合作 Stephen Fesik博士基于片段的NMR方法）。 互补的CS资源提供了应用先进的化学合成和药物的能力。 化学方法学，包括溶液相平行合成，微波辅助催化， 自动化质量定向纯化，以及手性合成和纯化资源。 HTS/CS雇用了一批训练有素的HTS专家，他们直接与范德比尔特调查人员合作， 开发、实施和解释HTS。该团队与CS设施中的团队携手合作， 研究人员通过提供一种使用药物的机制， 化学技术，以了解，改善和利用高温超导命中。大卫韦弗，博士，指导HTS 组件和Alex Waterson博士，引导CS部分。

The High Throughput Screening (HTS) and Chemical Synthesis (CS) Shared Resource provides state-ofthe- art capability for Vanderbilt-lngram Cancer Center (VICC) researchers to harness the power of HTS/CS to support target discovery, pathway mapping, chemical tool discovery, and exploratory preclinical drug discovery research. HTS/CS includes a chemical library of >170,0000 small molecules, advanced liquid handling, and a broad array of signal detecfion modalifies enabling researchers to address quesfions spanning from protein-protein interactions in purified cell-free systems to colony formation and morphology assays using automated imaging. The facility has the capability of addressing all commonly used opfical techniques (fluorescence, luminscense, kinefic imaging, high-content imaging, scinfillation proximity, refractive index spectroscopy (Corning Epic), automated electrophysiology, and very shortly in collaboration with Stephen Fesik, Ph.D. fragment-based NMR approaches). The complementary CS resource provides an ability to apply advanced chemical synthesis and medicinal chemistry methodologies including solution-phase parallel synthesis, microwave assisted catalysis, automated mass-directed purification, and both chiral synthesis and purificafion resources. HTS/CS employs a highly trained staff of HTS experts who work directly with Vanderbilt investigators to develop, implement, and interpret HTS. This team works hand-in-hand with the team in the CS facility to help investigators extract maximum value from the shared resource by providing a mechanism to use medicinal chemistry techniques to understand, improve, and ufilize HTS hits. David Weaver, Ph.D., directs the HTS component and Alex Waterson, Ph.D., directs the CS portion.