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Signaling to Cell Intercalation in Arabidopsis

拟南芥中细胞嵌入的信号转导

基本信息

批准号：
7682887
负责人：
Zhenbiao Yang
金额：
$ 28.09万
依托单位：
UNIVERSITY OF CALIFORNIA RIVERSIDE
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-01 至 2011-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7682887
关键词：
Actins Animals Appearance Arabidopsis Auxins Award Biochemical Biochemical Genetics Biological Biological Assay Biological Models Candidate Disease Gene Cells Complex Cytoskeleton Data Development Embryo Embryonic Development Epidermis Event Failure Genetic Goals Grant Growth Guanine Nucleotide Exchange Factors Guanosine Triphosphate Phosphohydrolases Health Hormones Human Image Intention Investigation Knock-out Knowledge Left Link Lobe Mass Spectrum Analysis Mediating Methods Microtubules Molecular Molecular Cloning Morphogenesis Neural Tube Defects Organism Organogenesis Pathway interactions Perception Phosphotransferases Plant Leaves Plant Model Plants Pregnancy Process Protein Kinase Proteins RIPK2 gene Receptor Signaling Regulation Research Research Personnel Role Shapes Signal Pathway Signal Transduction Signaling Molecule System Testing Time Tissues United States National Institutes of Health Work Yang base developmental disease gastrulation insight intercalation mutant novel plant growth/development programs receptor rho rho GTP-Binding Proteins spatiotemporal

项目摘要

The long-term goal of this project is to understand the underlying signaling mechanisms of cell intercalation, in which neighboring cells change their shapes to become intercalated with each other. Cell intercalation is critical for the proper development and morphogenesis of multi-cellular organisms. Cell intercalation is required for the process of convergent extension during gastrulation, neurulation, axis elongation, and organogenesis of animal and human embryos. However, signals and pathways governing this fundamental process are poorly characterized, though some underlying signaling events (e.g., Rho GTPases and the cytoskeleton) are known to be conserved across animal and plant kingdoms. In the model plant Arabidopsis, cell intercalation is important for the development of the leaf epidermis, in which pavement cells develop intercalary lobes and indentations to form the jigsaw-puzzle appearance. The Pi's group has developed the pavement cell as a model system for cell intercalation, and has established the first framework of a Rho GTPase-dependent intracellualr signaling network that controls this process. It is composed of two counteracting pathways: a ROP2-RIC4-actin pathway activating lobe formation and a ROP6-RIC1- microtubule pathway promoting indentation. In this project, signals, receptors and new components will be determined and linked to this framework. In aim 1, signaling events activating the ROP2 GTPase, e.g., signaling by the SPK1 ROP guanine nucleotide exchange factor, will be investigated using biochemical, genetic, and cell biological methods. In aim 2, molecules upstream of the ROP6 GTPase will be determined using similar approaches, and the intercellular signaling between the ROP6 and the ROP2 pathways will be investigated to understand how lobing and indenting are coordinated between the neighboring cells. Aim 3 is to investigate how auxin gradients, acting as a developmental signal, induce intercalary growth. A combination of rop mutants and biochemical and cell biological assays will be used to determine whether auxin activates the ROP2 or ROP6 pathway. The TMK receptor-like kinases that are required for intercalary growth will be tested for their participation in auxin perception. From these aims, a comprehensive picture of the molecular and cellular mechanisms for intercalary growth will be revealed. Given the conservation of the signaling mechanisms underlying cell intercalation across plants and humans, the knowledge gained from this research may provide new insights into our understanding of convergent extension. Because failure in convergent extension causes neural tube defects (NTDs), a common developmental disorder (1 out of 1000 pregnancies), this research is relevant to human health improvements. ^_^^

这个项目的长期目标是了解细胞插入的潜在信号机制，