Core C

核心C.蛋白质生产核心 导演：安振宇，博士 蛋白质生产核心将主要负责生产大量均匀、纯净的镁 (&gt；95%)病毒编码的蛋白质、人类细胞伙伴及其复合体，用于结构研究。 其次，核心将支持对这些蛋白质的生物物理和生物分析表征研究，并 蛋白质复合体有助于精炼蛋白质的原子和分子测定的生化基础 和情结。生产正确折叠和单分散的溶液尤为重要 材料。核磁共振或结晶学所需的纯化蛋白质的数量和性质 在规划结构生物学项目时，结构确定经常被低估。因此，我们， 高度重视通过PCHPI提供高质量的分析材料。为此，一个 提出了如下的一般方法。

Core C. Protein Production Core Director: Jinwoo Ahn, PhD The Protein Production Core will be primarily responsible for producing mg quantities of homogenous, pure (>95%) virus encoded proteins, human cellular partners, and their complexes for structural studies. Secondarily, the core will support biophysical and bioanalytical characterization studies of these proteins and protein complexes to aid in refining the biochemical bases for atomic and molecular determination of proteins and complexes. It is particularly important to produce correctly folded and mono-dispersed solutions of the material. The amounts and properties of purified proteins that are necessary for NMR or crystallographic structure determination are frequently underestimated when planning structural biology projects. We, therefore, place strong emphasis on providing high quality material for analysis through the PCHPI. To this end, a general approach as follows is proposed.