Core C

核心C

• 批准号: 8324239
• 负责人: jinwoo ahn
• 金额: $ 82.34万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8324239
• 关键词: Affinity; Affinity Chromatography; Biochemical; Biological Process; Cells; Chimeric Proteins; Circular Dichroism; Cloning; Code; Complementary DNA; Complex; Cytolysis; Doctor of Philosophy; Ensure; Escherichia coli; Eukaryota; Europe; Evolution; Excision; Fluorescence Spectroscopy; Gel Chromatography; Green Fluorescent Proteins; HIV; Human; In Vitro; Insecta; Isotope Labeling; Label; Laboratories; Ligands; Lower Organism; Mass Spectrum Analysis; Methodology; Methods; Molecular; Mono-S; Mutagenesis; Post-Translational Protein Processing; Process; Production; Property; Proteins; Proteomics; Protocols documentation; Recombinant Proteins; Reporter; Reproducibility; Sampling; Screening procedure; Selenomethionine; Site; Solubility; Solutions; Stream; Structure; System; Temperature; Testing; Thioredoxin; Thrombin; Time; Twin Multiple Birth; Universities; Variant; Virus; Western Blotting; X-Ray Crystallography; Yeasts; base; citrate carrier; expression cloning; expression vector; nephelometry; protein complex; protein expression; structural biology; structural genomics; success; vector

Core C. Protein Production Core Director: Jinwoo Ahn, PhD The Protein Production Core will be primarily responsible for producing mg quantities of homogenous, pure (>95%) virus encoded proteins, human cellular partners, and their complexes for structural studies. Secondarily, the core will support biophysical and bioanalytical characterization studies of these proteins and protein complexes to aid in refining the biochemical bases for atomic and molecular determination of proteins and complexes. It is particularly important to produce correctly folded and mono-dispersed solutions of the material. The amounts and properties of purified proteins that are necessary for NMR or crystallographic structure determination are frequently underestimated when planning structural biology projects. We, therefore, place strong emphasis on providing high quality material for analysis through the PCHPI. To this end, a general approach as follows is proposed.

核C.蛋白质生产核心 导演：Jinwoo Ahn，博士 蛋白质生产核心将主要负责产生Mg量的同质，纯 （> 95％）病毒编码的蛋白质，人类细胞伴侣及其结构研究的复合物。 其次，核心将支持这些蛋白质和 蛋白质复合物有助于精炼蛋白质原子和分子测定的生化碱基 和复合物。正确折叠和单分散解决方案特别重要 材料。 NMR或晶体学所需的纯化蛋白质的量和特性 规划结构生物学项目时，经常低估结构的确定。因此，我们 强烈强调通过PCHPI提供高质量的材料进行分析。为此， 一般方法如下。