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Core C

核心C

基本信息

批准号：
8324239
负责人：
jinwoo ahn
金额：
$ 82.34万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8324239
关键词：
Affinity Affinity Chromatography Biochemical Biological Process Cells Chimeric Proteins Circular Dichroism Cloning Code Complementary DNA Complex Cytolysis Doctor of Philosophy Ensure Escherichia coli Eukaryota Europe Evolution Excision Fluorescence Spectroscopy Gel Chromatography Green Fluorescent Proteins HIV Human In Vitro Insecta Isotope Labeling Label Laboratories Ligands Lower Organism Mass Spectrum Analysis Methodology Methods Molecular Mono-S Mutagenesis Post-Translational Protein Processing Process Production Property Proteins Proteomics Protocols documentation Recombinant Proteins Reporter Reproducibility Sampling Screening procedure Selenomethionine Site Solubility Solutions Stream Structure System Temperature Testing Thioredoxin Thrombin Time Twin Multiple Birth Universities Variant Virus Western Blotting X-Ray Crystallography Yeasts base citrate carrier expression cloning expression vector nephelometry protein complex protein expression structural biology structural genomics success vector

项目摘要

Core C. Protein Production Core Director: Jinwoo Ahn, PhD The Protein Production Core will be primarily responsible for producing mg quantities of homogenous, pure (>95%) virus encoded proteins, human cellular partners, and their complexes for structural studies. Secondarily, the core will support biophysical and bioanalytical characterization studies of these proteins and protein complexes to aid in refining the biochemical bases for atomic and molecular determination of proteins and complexes. It is particularly important to produce correctly folded and mono-dispersed solutions of the material. The amounts and properties of purified proteins that are necessary for NMR or crystallographic structure determination are frequently underestimated when planning structural biology projects. We, therefore, place strong emphasis on providing high quality material for analysis through the PCHPI. To this end, a general approach as follows is proposed.

核心C。蛋白质生产核心导演：Jinwoo Ahn，PhD 蛋白质生产核心将主要负责生产mg数量的均匀，纯（>95%）病毒编码蛋白、人类细胞伴侣及其复合物用于结构研究。其次，核心将支持这些蛋白质的生物物理和生物分析表征研究，蛋白质复合物，以帮助精炼蛋白质原子和分子测定的生化基础和复合物。特别重要的是，制备正确折叠的和单分散的本发明化合物的溶液。材料纯化蛋白质的数量和性质是核磁共振或晶体学所必需的在规划结构生物学项目时，结构确定常常被低估。因此，非常重视通过PCHPI提供高质量的分析材料。为此，一提出了如下的一般方法。