VISCERAL FAT CONTRIBUTION TO VERY LOW DENSITY LIPOPROTEIN (VLDL)-TRIGLYCERIDE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 男性和女性在脂肪代谢方面存在差异。男性的体重往往在腹部增加，而女性的体重则倾向于在臀部增加。在之前的一项研究中，我们发现，在健康瘦削的男性中，腹内脂肪组织对血浆甘油三酯的贡献约为25%。这一点在女性身上还没有确定。血浆甘油三酯浓度升高是冠心病的危险因素。我们假设，来自不同来源的脂肪对女性的血脂有贡献。本研究的目的是确定不同脂肪来源对女性血脂的贡献，尤其是胃部较深层的脂肪组织。这项研究包括输注标记棕榈酸酯和醋酸酯，并结合采血。最多15名18-40岁的女性将被招募。之后，10名受试者将完成24小时输液研究。输液研究包括放置两根导管，每只手臂一根，采集血液样本，将稳定的醋酸盐同位素注入无菌盐水中，以及与白蛋白结合的脂肪酸。在实验过程中将抽取大约250毫升的血液。通过在高度精密的仪器中研究血液样本，以测量血液中少量稳定同位素的水平，我们将能够计算出不同来源的脂肪对血液中甘油三酯的贡献。这项研究将有助于了解不同来源的甘油三酯在肝脏中产生的重要性，从而对女性血浆甘油三酯的重要性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Men and women differ in fat metabolism. Men tend to put on weight in the stomach region, whereas women tend to put on weight on their hips. In a previous study we found that the contribution of intra-abdominal fat tissue to plasma triglycerides was about 25% in healthy lean men. This has not been determined in women. Elevated plasma triglyceride concentration is a risk factor for coronary-heart diseases. We hypothesize that fat from different sources contribute to blood lipids in women. The purpose of this study is to determine the contribution from different fat sources, and especially the deeper fat tissue in the stomach region, to blood lipids in women. The study includes infusion of labeled palmitate and acetate combined with blood sampling. Up to fifteen women, 18-40 years, will be recruited. Ten subjects will thereafter complete a 24 hour infusion study. The infusion study involves the placement of two catheters, one in each arm, the taking of blood samples, infusion of stable isotopes of acetate in sterile saline and fatty acids bound to albumin. About 250 ml of blood will be drawn during the experiment. By studying the blood samples in highly sophisticated instruments to measure the small levels of stable isotopes in the blood, we will be able to calculate the contributions of fat from different sources to triglycerides in the blood. This study will lead to knowledge about the importance of different sources for triglyceride production in the liver and thus for plasma triglycerides in women.