VISCERAL FAT CONTRIBUTION TO VERY LOW DENSITY LIPOPROTEIN (VLDL)-TRIGLYCERIDE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 男性和女性在脂肪代谢方面存在差异。男性往往在腹部增加体重，而女性往往在臀部增加体重。在以前的研究中，我们发现，在健康的瘦男性中，腹内脂肪组织对血浆甘油三酯的贡献约为25%。这在妇女中尚未确定。血浆甘油三酯浓度升高是冠心病的危险因素。我们假设，不同来源的脂肪有助于女性血脂。本研究的目的是确定不同脂肪来源，特别是胃部深层脂肪组织对女性血脂的影响。该研究包括输注标记的棕榈酸酯和乙酸酯，并结合血液采样。将招募15名18至40岁的妇女。此后，10名受试者将完成24小时输注研究。输注研究包括放置两个导管，每组一个，采集血液样本，输注无菌盐水中的乙酸盐稳定同位素和与白蛋白结合的脂肪酸。实验期间将抽取约250 ml血液。通过在高度复杂的仪器中研究血液样本以测量血液中稳定同位素的少量水平，我们将能够计算来自不同来源的脂肪对血液中甘油三酯的贡献。这项研究将导致知识的重要性，不同来源的甘油三酯生产在肝脏中，从而为血浆甘油三酯的妇女。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Men and women differ in fat metabolism. Men tend to put on weight in the stomach region, whereas women tend to put on weight on their hips. In a previous study we found that the contribution of intra-abdominal fat tissue to plasma triglycerides was about 25% in healthy lean men. This has not been determined in women. Elevated plasma triglyceride concentration is a risk factor for coronary-heart diseases. We hypothesize that fat from different sources contribute to blood lipids in women. The purpose of this study is to determine the contribution from different fat sources, and especially the deeper fat tissue in the stomach region, to blood lipids in women. The study includes infusion of labeled palmitate and acetate combined with blood sampling. Up to fifteen women, 18-40 years, will be recruited. Ten subjects will thereafter complete a 24 hour infusion study. The infusion study involves the placement of two catheters, one in each arm, the taking of blood samples, infusion of stable isotopes of acetate in sterile saline and fatty acids bound to albumin. About 250 ml of blood will be drawn during the experiment. By studying the blood samples in highly sophisticated instruments to measure the small levels of stable isotopes in the blood, we will be able to calculate the contributions of fat from different sources to triglycerides in the blood. This study will lead to knowledge about the importance of different sources for triglyceride production in the liver and thus for plasma triglycerides in women.