PILOT STUDY OF DCA EFFECTS ON HUMAN TYROSINE METABOLISM

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们建议对3名健康成人进行概念验证性研究，他们将在12.5 mg/kg/biDCA(典型的临床剂量和剂量程序)之前和5天后静脉注射13C-U-酪氨酸。我们将通过我们以前使用的标准动力学建模技术以及我们开发的用于鉴定和定量酪氨酸和DCA及其代谢物的GC/MS和LC/MS方法来确定酪氨酸动力学。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose to conduct a proof-of-concept investigation in 3 healthy adults who would receive 13C-U-tyrosine by vein before and after 5 days of 12.5 mg/kg/bid DCA, the typical clinical dose and dose schedule. We will determine tyrosine kinetics by standard kinetic modeling techniques we have employed previously and by GC/MS and LC/MS methods we have developed to identify and quantify both tyrosine and DCA and their metabolites.