RNA干扰-猫眼课题宝

RNA干扰

批准号：

7694421

负责人：

Jianzhu Chen

金额：

$42.79万

依托单位：

MASSACHUSETTS INSTITUTE OF TECHNOLOGY

依托单位国家：

美国

项目类别：

财政年份：

资助国家：

美国

项目状态：

未结题

起止时间：

至

来源：

https://reporter.nih.gov/project-details/7694421

关键词：

Address Affect Algorithms Animals Area Base Sequence Biological Assay Biological Process Biology Boston Cancer Center Cells Characteristics Chromatin Collaborations Collection Colorado Communities Complement Complex Computer Simulation Condition Contracts Cultured Cells DNA DNA Polymerase II DNA Polymerase III DNA Sequence DNA delivery Data Databases Development Ensure Fees Fostering Gene Expression Gene Silencing Gene Targeting Generations Genes Genetic Genetic Screening Genetic Transcription Genomics Goals Human In Vitro Individual Invasive Laboratories Lentivirus Vector Libraries Liver Luciferases Lung Malignant Neoplasms Mammalian Cell Mammals Mediating Mediation Medical Messenger RNA Methodology Methods MicroRNAs Molecular Genetics Mus New Jersey Nucleic Acid Regulatory Sequences Numbers Oligonucleotides Organ Participant Plasmids Polyethyleneimine Polymerase Polymerase Chain Reaction Polymers Procedures Process Production Proteins Protocols documentation Publishing RNA RNA Interference RNA Sequences Range Reagent Reporter Research Research Institute Research Personnel Research Project Grants Resources Retroviridae Role Selection Criteria Services Site Small Interfering RNA Small RNA Solutions Specificity Stem cells Structure Subfamily lentivirinae System Systems Biology Technology Testing Tetracycline Tetracyclines Time Tissues Training Transcription Initiation Transfection Transgenic Animals Validation Work Workplace animal tissue base cell type chemical synthesis cost cost effectiveness desire discount expression vector gain of function gene function high throughput screening improved in vivo interest loss of function member novel novel strategies plasmid DNA programs promoter repository research study response skills small hairpin RNA tool tumor progression vector web-accessible

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中文摘要

翻译

这一提议的一个关键方面，嵌入在三个研究项目中，是验证由涉及癌症进展的特定细胞程序的计算模型所做的预测。这些预测需要通过功能获得或功能丧失实验在细胞和动物身上进行验证。

英文摘要

A key aspect of this proposal, embedded in each of the three research projects is the validation of predictions made by computational models of specific cellular programs involve in cancer progression. Those predictions need to be tested in cells and in animals by gain-of-function or loss of-function experiments. RNA interference (RNAi) has emerged as a novel cellular mechanism regulating gene expression at the posttranscriptional level and as a powerful tool to control gene function experimentally There have been several recent advances in the biology and application of RNAi. These include the definition of improved criteria for selecting effective small interfering RNA (siRNA) sequences ; improved methods for delivery of short interfering RNAs (siRNAs) or expression of short hairpin RNAs (shRNAs), which in turn generate siRNAs leading to stable silencing of genes in mammalian cells, tissues, and animals using retroviral expression systems. Both focused and high-throughput screening projects based on RNAi have been initiated to search for genes involved in basic biological processes, as well as in complex pathological conditions associated with cancer. The ICBP RNAi resource will develop new siRNA/shRNA delivery technologies and will provide support to the participants in this program that plan to use RNAi technologies in their research. The specific aims of the RNAi resource facility are to: 1. Generate and distribute a set of functionally validated siRNAs and shRNAs that are able to silence genes of interest to the ICBP. 2. Develop and improve selection criteria for functional siRNA sequences. 3. Develop new approaches to achieve constitutive, regulated, or tissue-specific delivery of siRNAs and expression of shRNAs. 4. Provide technical and scientific advice to ICBP investigators on how to use RNAi technologies.

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