Core Laboratory for Neuromolecular Production

描述（由申请人提供）：该申请将建立神经分子生产核心实验室（CLNP），为神经科学研究人员提供定制的小分子工具，咨询，资源和支持，以协助和推进他们的研究。神经分子生产核心实验室将为神经科学研究人员建立和维护必要的化学支持，包括小分子试剂，探针和/或靶点的制备，优化，纯化和/或分离，重点是基于神经递质的结构。CLNP将进行生物活性配体，交联剂，同位素富集结构，肽，结构和成像探针，蛋白质标签和生物缀合物，文库开发（靶向和多样性导向合成）和其他分子集合的合成。CLNP将通过增加新的空间，创建专用的CLNP实验室和程序套件，以团队合作为导向的管理，基于工作组的问题解决，与现有核心的集成以及聘请训练有素的化学人员来设计，维护和执行所要求的项目和服务来实现。长期目标是将CLNP作为神经科学研究人员的可靠和可持续的服务和资源，引入能够产生新的研究途径的小分子档案（仓库）和化学信息学数据库。本申请的具体目的是：（1）设计，建造和启动神经分子生产核心实验室，（2）为神经科学研究人员提供定制的基于神经递质的配体，探针，生物缀合物和资源，以帮助和加强他们现有的，待定的和计划的基于NINDS的研究计划，（3）开发工作组，促进合作和使用基于神经递质的配体，探针和生物缀合物解决多实验室问题，以及（4）创建，管理和传播化学信息学数据库和基于神经递质的配体、探针和生物缀合物的储存库供公众使用。

DESCRIPTION (provided by applicant): This application will establish a Core Laboratory in Neuromolecular Production (CLNP) that provides neuroscience researchers with customized small molecule tools, consultation, resources and support to assist and advance their investigations. The Core Laboratory in Neuromolecular Production will establish and maintain essential chemistry support for neuroscience investigators including the preparation, optimization, purification and/or separation of small molecule reagents, probes and/or targets that are focused on neurotransmitter-based structures. The CLNP will undertake the synthesis of bioactive ligands, cross-linkers, isotope enriched structures, peptides, structure and imagine probes, protein-tags and bioconjugates, library development (target- and diversity-oriented syntheses), and other molecular ensembles. The CLNP will be achieved with the addition of new space, creation of a dedicated CLNP lab and program suite, a teamwork-oriented administration, workgroup-based problem solving, integration with existing cores, and the hiring of highly trained chemistry personnel to design, maintain, and execute the requested projects and services. The long-range objectives are to advance the CLNP as a dependable and sustainable service and resource to neuroscience investigators that introduces small molecule archives (warehouse) and chemoinformatic databases capable of generating new research avenues. The specific aims of this application are to: (1) design, build, and launch a Core Laboratory in Neuromolecular Production, (2) provide neuroscience investigators with customized neurotransmitter-based, ligands, probes, bioconjugates and resources to aid and enhance their existing, pending and planned NINDS-based research programs, (3) develop workgroups that promote collaborations and multi-laboratory problem solving using neurotransmitter-based ligands, probes and bioconjugates, and (4) create, manage and disseminate for public use a cheminformatic database and repository of neurotransmitter-based ligands, probes and bioconjugates.