Core Laboratory for Neuromolecular Production

该申请将建立一个神经分子生产核心实验室（CLNP）， 神经科学研究人员提供定制的小分子工具，咨询，资源和支持， 协助和推动他们的调查。神经分子生产核心实验室将建立 并为神经科学研究人员提供必要的化学支持，包括准备， 优化、纯化和/或分离小分子试剂、探针和/或靶标， 专注于基于神经递质的结构CLNP将进行生物活性配体的合成， 交联剂、同位素富集结构、肽、结构和成像探针、蛋白质标签和 生物缀合物、文库开发（靶向和多样性导向的合成）和其他分子生物学方法。 合奏。CLNP将通过增加新空间，创建专用CLNP实验室来实现 和程序套件，面向团队工作的管理，基于工作组的问题解决，与 现有的核心，并聘请训练有素的化学人员来设计，维护和执行 要求的项目和服务。长期目标是将CLNP作为一个可靠的 为神经科学研究人员提供可持续的服务和资源，介绍小分子档案 （仓库）和化学信息学数据库能够产生新的研究途径。具体 该申请的目的是：（1）设计、建造和启动神经分子生产核心实验室， (2)为神经科学研究人员提供定制的基于神经递质的配体，探针， 生物缀合物和资源，以帮助和加强他们现有的，悬而未决的和计划的NINDS为基础的研究 计划，（3）开发工作组，促进合作和多实验室解决问题，使用 基于神经递质的配体、探针和生物缀合物，以及（4）创建、管理和传播 公众使用化学信息学数据库和基于神经递质的配体、探针和 生物缀合物。 公共卫生相关性：该应用程序旨在开发一种设施， 分子到神经科学家。小分子开发是开发药物的重要第一步 可能有助于对抗中枢神经系统疾病。

This application will establish a Core Laboratory in Neuromolecular Production (CLNP) that provides neuroscience researchers with customized small molecule tools, consultation, resources and support to assist and advance their investigations. The Core Laboratory in Neuromolecular Production will establish and maintain essential chemistry support for neuroscience investigators including the preparation, optimization, purification and/or separation of small molecule reagents, probes and/or targets that are focused on neurotransmitter-based structures. The CLNP will undertake the synthesis of bioactive ligands, cross-linkers, isotope enriched structures, peptides, structure and imagine probes, protein-tags and bioconjugates, library development (target- and diversity-oriented syntheses), and other molecular ensembles. The CLNP will be achieved with the addition of new space, creation of a dedicated CLNP lab and program suite, a teamwork-oriented administration, workgroup-based problem solving, integration with existing cores, and the hiring of highly trained chemistry personnel to design, maintain, and execute the requested projects and services. The long-range objectives are to advance the CLNP as a dependable and sustainable service and resource to neuroscience investigators that introduces small molecule archives (warehouse) and chemoinformatic databases capable of generating new research avenues. The specific aims of this application are to: (1) design, build, and launch a Core Laboratory in Neuromolecular Production, (2) provide neuroscience investigators with customized neurotransmitter-based, ligands, probes, bioconjugates and resources to aid and enhance their existing, pending and planned NINDS-based research programs, (3) develop workgroups that promote collaborations and multi-laboratory problem solving using neurotransmitter-based ligands, probes and bioconjugates, and (4) create, manage and disseminate for public use a cheminformatic database and repository of neurotransmitter-based ligands, probes and bioconjugates. Public Health Relevance: This application seeks to develop a facility that provides customized small molecules to neuroscientists. Small molecule development is an essential first step toward developing drugs that may be helpful in battling CNS diseases.