DISPOSITION, METABOLISM & PROCESSING OF OPIATE AGONISTS, ANTAGONISTS & OPIOIDS

性格、新陈代谢

• 批准号: 7722184
• 负责人: MARY J KREEK
• 金额: $ 0.66万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722184
• 关键词: Agonist; Cholecystokinin; Cocaine; Computer Retrieval of Information on Scientific Projects Database; Corticotropin; Corticotropin-Releasing Hormone; Dopamine; Dynorphin A; Dynorphins; Enkephalins; Fluoxetine; Funding; Grant; High Pressure Liquid Chromatography; Hormones; Institution; Mass Spectrum Analysis; Metabolism; Methadone; Methods; Morphine; Narcotic Antagonists; Neuropeptides; Neurotransmitters; Opiates; Opioid Peptide; Parents; Peptides; Pro-Opiomelanocortin; Process; Proteins; Radioimmunoassay; Research; Research Personnel; Resources; Source; Substance P; System; Techniques; United States National Institutes of Health; alcohol effect; beta-Endorphin; drug of abuse; endogenous opioids; extracellular; gamma-MSH; nalmefene; novel; prodynorphin; proenkephalin; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The specific aims are A. To develop novel methods using mass spectrometry to qualitatively (and subsequently, semi-quantitatively or quantitatively) determine the presence of multiple neuropeptides derived from a single gene product (e.g. prodynorphin and processed dynorphin A peptides; proenkephalin and processed enkephalin peptides; proopiomelanocortin (POMC) parent peptide and multiple peptides derived from POMC, adrenocorticotropin hormone (ACTH), beta-endorphin, of gamma-melanocyte-stimulating hormone (MSH). B.To use these novel techniques (alone or in combination with HPLC and/or RIA) for the simultaneous qualitative and/or quantitative analysis of opioid peptides and other neuropeptides related to the endogenous opioid system (e.g. Corticotropin releasing factor (CRF), cholecystokinin (CCK), substance P and their processed peptides). C. To use these techniques in studies of the effects of alterations in levels of activity of neurotransmitters on the processing of endogenous opioids (e.g. the effects of altered levels of dopamine on dynorphin processing). D. To use these techniques to study the effects of drugs of abuse and potential treatment agents on opioid peptide processing and degradation (e.g. cocaine, morphine and ethanol effects; methadone, nalmefene and fluoxetine effects). We are currently developing novel MS tools for determining specific endogenous extracellular neuropeptides.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 具体目标是A.开发新的方法，使用质谱定性（并且随后，半定量或定量地）确定来源于单个基因产物的多种神经肽的存在（例如，强啡肽原和加工的强啡肽A肽;脑啡肽原和加工的脑啡肽;阿黑皮素原（POMC）母体肽和衍生自POMC的多种肽，促肾上腺皮质激素（ACTH），β-内啡肽，伽马黑素细胞刺激激素（MSH）。 B.将这些新技术（单独或与HPLC和/或RIA结合）用于同时定性和/或定量分析阿片肽和与内源性阿片系统相关的其他神经肽（例如促肾上腺皮质激素释放因子（CRF）、胆囊收缩素（CCK）、P物质及其加工肽）。 C.使用这些技术研究神经递质活性水平的改变对内源性阿片类物质加工的影响（例如多巴胺水平的改变对强啡肽加工的影响）。 D.使用这些技术研究滥用药物和潜在治疗药物对阿片肽加工和降解的影响（例如可卡因、吗啡和乙醇的影响;美沙酮、纳美芬和氟西汀的影响）。 我们目前正在开发新的MS工具，用于确定特定的内源性细胞外神经肽。