BTSL

BTSL

• 批准号: 7959155
• 负责人: DOROTHEA PARKER
• 金额: $ 13.18万
• 依托单位: MOREHOUSE SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959155
• 关键词: Animals; Antibodies; Antibody Formation; Antigens; Back; Biomedical Research; Biomedical Technology; Cell Separation; Charge; Clinical Sciences; Cloning; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Cytometry; Equipment; Faculty; Funding; Grant; Hemorrhage; Human Resources; Immunization; Institution; Instruction; Laboratories; Left; Liquid substance; Location; Monoclonal Antibodies; Mus; New Zealand; Nitrogen; Oryctolagus cuniculus; Price; Proteins; Reporting; Research; Research Personnel; Resources; Schools; Serum; Services; Source; Students; Techniques; Time; United States National Institutes of Health; base; chemokine; cost; cytokine; instrumentation; medical schools; monoclonal antibody production; polyacrylamide gels; polyclonal antibody; protocol development; synthetic peptide

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dorothea V. Parker, Leader The Biomedical Technology Service Laboratory (BTSL) at Morehouse School of Medicine has been in existence for twenty years. During this time, the BTSL has enhanced and supported the biomedical research of the basic and clinical science faculty at the institution tremendously. This support extends to the personnel in their laboratories as well as graduate and undergraduate students from the medical school and neighboring AUC institutions. The BTSL is the largest core facility at the institution occupying eight laboratory locations on the main campus. It provides consistent support for all types of multi-use equipment, research necessities, techniques, group as well as one on one instrumentation instruction and protocol development. The BTSL also currently offers the specialized services of monoclonal and polyclonal antibody production. Monoclonal antibodies are produced using Balb/c mice. Polyclonal antibodies are produced using New Zealand white rabbits. The polyclonal and monoclonal core makes antibodies against conjugated synthetic peptides, soluble proteins,0 chemokines, cytokines, cellular antigens, protein bands excised from polyacrylamide gels plus a variety of other antigens. The Cytometry and Cell Sorting Function was placed back under the BTSL. The technician, Dr Udai Singh, for the Cytometry and Cell Sorting Function left the school in late 2007/early 2008. Additionally, the equipment, which was very old is now essentially down. Service Charges: Service charges have remained the same as those listed in last year's report. We are currently assessing and considering new charge amounts. 1) Liquid Nitrogen $0.85/liter 2)Monoclonal Antibody Production $1,500.00 (includes animal costs, inoculations, fusion and cloning) The price for outside investigators is $2,000.00. 3) Polyclonal Antibody Production charges are based on the number of rabbits, immunizations, bleeds and the serum amount required.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Dorothea V.帕克，领袖 莫尔豪斯医学院的生物医学技术服务实验室（BTSL）已经存在了20年。在此期间，BTSL极大地增强和支持了该机构基础和临床科学教师的生物医学研究。这种支持延伸到他们的实验室人员以及医学院和邻近AUC机构的研究生和本科生。BTSL是该机构最大的核心设施，占据了主校区的八个实验室位置。它为所有类型的多用途设备、研究必需品、技术、组以及一对一仪器说明和协议开发提供一致的支持。 BTSL目前还提供单克隆和多克隆抗体生产的专业服务。使用Balb/c小鼠产生单克隆抗体。使用新西兰白色兔产生多克隆抗体。多克隆和单克隆核心产生针对缀合的合成肽、可溶性蛋白质、趋化因子、细胞因子、细胞抗原、从聚丙烯酰胺凝胶上切下的蛋白质条带以及各种其他抗原的抗体。 将细胞计数和细胞分选功能放回BTSL下。细胞计数和细胞分选功能的技术员Udai Singh博士于2007年底/2008年初离开学校。此外，设备，这是非常旧的，现在基本上是下降。 服务费用： 服务费与去年报告中所列的服务费相同。我们目前正在评估和考虑新的收费标准。 1)液氮$0.85/升 2)单克隆抗体生产$1，500.00（包括动物成本，接种，融合和克隆）外部研究者的价格为$2，000.00。 3)多克隆抗体生产费用基于家兔数量、免疫接种、采血和所需血清量。