BTSL

BTSL

• 批准号: 8166167
• 负责人: DOROTHEA PARKER
• 金额: $ 15.38万
• 依托单位: MOREHOUSE SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8166167
• 关键词: Antibodies; Antibody Formation; Antigens; Biomedical Research; Biomedical Technology; Clinical Sciences; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Dry Ice; Equipment; Faculty; Funding; Grant; Human Resources; Institution; Instruction; Laboratories; Laboratory Personnel; Liquid substance; Location; Monoclonal Antibodies; Mus; New Zealand; Nitrogen; Oryctolagus cuniculus; Proteins; Research; Research Personnel; Resources; Services; Source; Students; Techniques; Time; United States National Institutes of Health; Water; chemokine; cryogenics; cytokine; instrumentation; medical schools; polyacrylamide gels; polyclonal antibody; protocol development; synthetic peptide; working group

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Biomedical Technology Service Laboratory (BTSL) Lab Leader - Dorothea Parker Working Group - Dorothea Parker, Jonathan Stiles, James Lillard Description: The Biomedical Technology Service Laboratory (BTSL) at Morehouse School of Medicine has been in existence for twenty one years. During this time, the BTSL has enhanced and supported the biomedical research of the basic and clinical science faculty at the institution tremendously. This support extends to the personnel in their laboratories as well as graduate and undergraduate students from the medical school and neighboring AUC institutions. The BTSL is the largest core facility at the institution occupying eight laboratory locations on the main campus. It provides consistent, reliable support for small and large multi-use equipment, research necessities such as dry ice, liquid nitrogen, ultra-pure water, cryogenic and ultra low freezer space. The BTSL personnel provide group as well as one on one instrumentation instruction and support for technique/ protocol development. The BTSL also offers the specialized services of monoclonal and polyclonal antibody production. Monoclonal antibodies are produced using Balb/c mice. Polyclonal antibodies are produced using New Zealand white rabbits. The polyclonal and monoclonal core makes antibodies against conjugated synthetic peptides, soluble proteins, chemokines, cytokines, cellular antigens, protein bands excised from polyacrylamide gels plus a variety of other antigens.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 生物医学技术服务实验室（BTSL） 实验室负责人- Dorothea帕克 工作组- Dorothea帕克、Jonathan Stiles、James Lillard 描述：生物医学技术服务实验室（BTSL）在莫尔豪斯医学院已经存在了二十一年。在此期间，BTSL极大地增强和支持了该机构基础和临床科学教师的生物医学研究。这种支持延伸到他们的实验室人员以及医学院和邻近AUC机构的研究生和本科生。BTSL是该机构最大的核心设施，占据了主校区的八个实验室位置。它为小型和大型多用途设备提供一致、可靠的支持，研究必需品，如干冰、液氮、超纯水、低温和超低冷冻空间。 BTSL人员为技术/方案开发提供小组以及一对一的仪器说明和支持。 BTSL还提供单克隆和多克隆抗体生产的专业服务。使用Balb/c小鼠产生单克隆抗体。使用新西兰白色兔产生多克隆抗体。多克隆和单克隆核心产生针对缀合的合成肽、可溶性蛋白质、趋化因子、细胞因子、细胞抗原、从聚丙烯酰胺凝胶上切下的蛋白质条带以及各种其他抗原的抗体。