PRODUCTION OF SOMATIC CELL NUCLEAR TRANSFER EMBRYOS AND EMBRYONIC STEM CELLS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 利用体细胞核移植后的成年成纤维细胞和卵母细胞建立恒河猴胚胎干细胞系。 成人成纤维细胞用作核的供体，在原始卵母细胞核物质被去除后，将核转移到卵母细胞中。 然后可以激活SCNT卵母细胞并培养胚胎，使得内细胞团可以用于产生胚胎干细胞系。 能够从细胞中产生胚胎干细胞在再生医学中有巨大的应用，然而，在应用于人类医学之前，必须在非人类灵长类动物中研究这一过程。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To use the rhesus monkey model to produce embryonic stem cell lines from adult fibroblast cells and oocytes that have been treated with somatic cell nuclear transfer. Adult fibroblast cells are used as donors for nuclei that are transferred to oocytes after the original oocyte nuclear material has been removed. SCNT oocytes can then be activated and embryos cultured so that the inner cell mass can be used to generate an embryonic stem cell line. Being able to produce embryonic stem cells from cells has tremendous applications in regenerative medicine, however, the procedures for this must be investigated in a nonhuman primate before being applied to human medicine.