Creation of a Human Recombinant Polyclonal Antibody therapy against Clostridium d

描述（由申请人提供）：该项目的目标是创建一种针对艰难梭菌相关疾病（CDAD）的人重组多克隆抗体（HRPA）疗法。在过去的几十年里，艰难梭菌已经成为美国、加拿大和欧洲医院内腹泻的最重要原因。以前的一线抗生素治疗对新出现的抗生素耐药菌株越来越无效，CDAD的死亡率正在上升。迫切需要新的治疗方案。本文提出的针对CDAD的HRPA治疗将包括针对疾病生物体的多种抗原和表位的几种抗体。HRPA疗法的发展是一个复杂的、多阶段的过程。第一阶段的目标是建立一个小但可行的人类抗艰难梭菌抗体文库，以证明在第二阶段研究中建立一个更完整的文库用于动物试验的可行性。

DESCRIPTION (provided by applicant): The goal of this project is to create a human recombinant polyclonal antibody (HRPA) therapy against C. difficile associated disease (CDAD). Over the last several decades, C. difficile has become the most important cause of nosocomial diarrhea in the U.S., Canada and Europe. Previously first-line antibiotic treatments are increasingly ineffective against emerging antibiotic-resistant strains and the mortality rate from CDAD is rising. New treatment options are desperately needed. The HRPA therapy for CDAD proposed here will consist of several antibodies targeting multiple antigens and epitopes of the disease organism. Development of an HRPA therapy is a complex, multi-stage process. The phase I goal is to create a small but viable library of human antibodies against C. difficile to demonstrate the feasibility of creating a more complete library in a phase II study for testing in animals. The phase I specific aims for this project are: 1. to recruit subjects from three groups of potential donors with known or likely exposure to C. difficile, obtain blood samples and isolate the plasma cells in order to clone the antibody genes; 2. to generate a test library of cloned and expressed antibodies using a subset of the plasma cells obtained from each donor; 3. to optimize and carry out an ELISA-based screen for antibodies recognizing C. difficile proteins in culture supernatant, bacterial lysate and toxins; and 4. to analyze the antibody library generated in aim 3 and determine whether additional screening will be required for the Phase II study. Following a successful Phase I, the goals of a Phase II study would be: 1. to analyze the antibody-epitope interactions of antibodies obtained in Phase I; 2. to perform large scale expression and purification of C. difficile antibodies; and 3. to carry out a series of animal studies to examine the effectiveness of several antibody cocktails in an animal model of CDAD. Following the successful completion of animal trials, Excelimmune will seek to bring a CDAD therapeutic product to market through strategic partnerships with larger biopharmaceutical companies. PUBLIC HEALTH RELEVANCE: C. difficile associated disease (CDAD) is a growing public health problem due to the rise of highly virulent, antibiotic resistant strains. According to the CDC, approximately 3 million cases of CDAD occur annually, with mortality rates of approximately 1-2.5%, and these numbers are on the rise. The overall goal of this project is to develop a novel therapeutic for CDAD, based on the use of cloned human antibodies that will effectively treat the disease and prevent the rise of resistant strains.