Development of a field deployable cell analyzer for the measurements of total lym

开发用于测量总淋巴细胞的现场可部署细胞分析仪

• 批准号: 7753134
• 负责人: Sherry Ann Dunbar
• 金额: $ 17.31万
• 依托单位: LUMINEX CORPORATION
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7753134
• 关键词: AIDS/HIV problem; Address; Africa South of the Sahara; Antibodies; Area; B-Lymphocytes; Biological; Blood specimen; CD4 Lymphocyte Count; CD4 Positive T Lymphocytes; Cells; Cessation of life; Child; Complex; Consumption; Country; Detection; Development; Diagnostic; Evaluation; Fingers; Government; HIV; Healthcare; Image; Immune system; Immunoassay; Individual; Infant; Leukocytes; Life; Lymphocyte; Magnetism; Malaria; Measurement; Monitor; Natural Killer Cells; Optics; Patients; Persons; Phase; Population; Research; Research Infrastructure; Research Proposals; Resources; Rest; Sampling; Small Business Innovation Research Grant; Specimen; System; T-Lymphocyte; Techniques; Technology; Vision; Whole Blood; base; cell analyzer; clinically relevant; cost; design; fluorescence imaging; innovation; magnetic field; pandemic disease; point-of-care diagnostics; prototype; public health relevance; tool

DESCRIPTION (provided by applicant): The proposed technology aims at creating a simple, low-cost and rugged cell analyzer capable of enumerating different populations of white blood cells, all lymphocytes, CD4 T-cells and the percentage of CD4 T-cells among all lymphocytes, directly from a small whole blood sample collected by finger prick. This technology is intended to be field deployable and to address the need for monitoring of HIV infected individuals, including children and infants, living in remote areas of resource-poor countries. A combination of techniques, including the immuno-magnetic capture and optical detection of biological cells will enable the isolation, identification and enumeration of T-Cells including the sub-population of CD4T-cells, B-Cells, and Natural Killer Cells directly from a complex sample matrix. PUBLIC HEALTH RELEVANCE: The proposed technology is designed to provide a low-cost, low-consumption and rugged field deployable diagnostic tool capable of isolating, identifying and enumerating biological cells directly from a small volume of specimen including whole blood. This new cytometer is ideally suited to respond to the current challenges of point-of-care diagnostics and to fill the gap that exists in regions with low health-care infrastructure such as in Sub-Saharan Africa where pandemic diseases like HIV/AIDS, TB and Malaria are the cause of million of deaths every year.

描述(申请人提供)：建议的技术旨在创造一种简单、低成本和坚固耐用的细胞分析仪，能够直接从指尖采集的一小部分全血样本中计数不同种类的白细胞、所有淋巴细胞、CD4T细胞和所有淋巴细胞中CD4T细胞的百分比。这项技术旨在可在外地部署，并满足对居住在资源贫乏国家偏远地区的艾滋病毒感染者，包括儿童和婴儿进行监测的需要。生物细胞的免疫磁性捕获和光学检测等技术的组合将能够直接从复杂的样本基质中分离、鉴定和计数T细胞，包括CD4T细胞、B细胞和自然杀伤细胞亚群。与公共卫生相关：拟议的技术旨在提供一种低成本、低消耗和坚固耐用的现场可部署诊断工具，能够直接从包括全血在内的少量样本中分离、鉴定和计数生物细胞。这种新的细胞仪非常适合于应对当前医疗保健点诊断的挑战，并填补卫生保健基础设施较低的地区存在的缺口，例如在撒哈拉以南非洲，艾滋病毒/艾滋病、结核病和疟疾等大流行疾病是每年数百万人死亡的原因。