STANDARD VALUES OF EOSINOPHIL-RELATED PARAMETERS FOR DATA COMPARISON

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 嗜酸性粒细胞是响应白细胞介素-3、-5（IL-3、IL-5）和粒细胞巨噬细胞集落刺激因子（GM-CSF）在骨髓中发育的免疫细胞。 成熟的嗜酸性粒细胞迁移到肠道，参与寄生虫免疫，特别是蠕虫。 嗜酸性粒细胞也与许多过敏性疾病有关，包括哮喘、特应性皮炎、药物反应和慢性荨麻疹。嗜酸性粒细胞相关的胃肠道疾病包括嗜酸性粒细胞性食管炎、嗜酸性粒细胞性胃肠炎和胶原性结肠炎。嗜酸性粒细胞增多综合征包括嗜酸性粒细胞显著增加的患者。嗜酸性粒细胞也参与掺假食品引起的疾病，如嗜酸性粒细胞增多症、肌痛综合征和毒油综合征。糖皮质激素已被用作许多这些疾病的治疗方法;然而，长期使用类固醇会导致显着的副作用。 此类测定的值只能放在有意义的背景下与正常患者值进行比较。 由于实验室间设备、技术和试剂的差异，文献值通常是不够的。 每个实验室都有必要建立测定的正常值。 此外，正常患者样本应经常与研究参与者样本一起运行，以确认设备保持校准且技术不变。 本项目的目标是验证设备是否始终校准和工作，以及 获得一系列正常值，用于比较当前和未来研究的患者样本。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Eosinophils are immune cells that develop in the bone marrow in response to interleukins-3, -5 (IL-3, IL-5) and granulocyte macrophage colony stimulating factor (GM-CSF). Mature eosinophils migrate to the gut and are involved in parasitic immunity, especially to helminthes. Eosinophils are also associated with many allergic diseases including asthma, atopic dermatitis, drug reactions and chronic urticaria. Eosinophil-associated gastrointestinal diseases include eosinophilic esophagitis, eosinophilic gastroenteritis and collagenous colitis. The hypereosinophilic syndrome includes patients with marked increases in eosinophils. The eosinophil is also involved in diseases caused by adulterated food products such as eosinophilia myalgia syndrome and toxic oil syndrome. Glucocorticoids have been used as a treatment for many of these diseases; however, long-term steroid usage causes significant side effects. Values for such assays can only be placed in meaningful context with comparison against normal patient values. Because of inter-laboratory variations in equipment, technique, and reagents, literature values will usually not suffice. It is necessary for each laboratory to establish normal values for the assays. Further, normal patient samples should be frequently run alongside study participant samples, in order to confirm that the equipment remains calibrated and technique unchanged. The objective of this project is to verify that equipment is consistently calibrated and working, as well as to obtain a range of normal values against which to compare patient samples for a number of present and future studies.