TMEM103 in Eosinophil Development

TMEM103 在嗜酸性粒细胞发育中的作用

• 批准号: 7660267
• 负责人: Gerald J Gleich
• 金额: $ 18.81万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-15 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7660267
• 关键词: Allergic; Amino Acid Sequence; Asthma; Atopic Dermatitis; Bone Marrow; Bone Marrow Cells; CD34 gene; Cells; Chronic; Development; Disease; Event; Extrinsic asthma; Genes; Genetic Transcription; Grant; Hematopoietic stem cells; Immune; Immunity; Immunohistochemistry; Integral Membrane Protein; Interleukin-5; Leukocytes; Location; Mass Spectrum Analysis; Messenger RNA; Methylcellulose; Microscopy; Monoclonal Antibodies; Morphology; Names; PAWR protein; Peptide Sequence Determination; Pharmaceutical Preparations; Production; Proteins; Quantitative Microscopy; RNA Interference; Reaction; Research; Reverse Transcriptase Polymerase Chain Reaction; Ribosomes; Role; Sedimentation process; Signal Transduction; Sucrose; Time; Tissues; Transcript; Translating; Urticaria; Western Blotting; density; eosinophil; mRNA Expression; novel; polyclonal antibody; public health relevance

DESCRIPTION (provided by applicant): Eosinophils are associated with many allergic diseases including asthma, atopic dermatitis, drug reactions and chronic urticaria. The myriad of eosinophil related diseases has led us to investigate the development of eosinophils from hematopoietic stem cells in an attempt to understand the signals prompting eosinophil production in the bone marrow. Gene profiling of developing eosinophils has shown that a novel hypothetical protein is expressed as mRNA. TMEM103 mRNA is expressed in IL-5 stimulated CD34+ cells, bone marrow and in mature eosinophils. The transcript, TMEM103, has been shown to be associated with ribosomes and the protein has been identified by western blot analysis in developing eosinophils. This grant proposes to further verify the existence of TMEM103 by raising monoclonal antibodies to the protein, then purifying and sequencing the protein. Further, the function of TMEM103 will be investigated by RNA silencing followed by studies investigating morphological and transcription profile changes. PUBLIC HEALTH RELEVANCE: Eosinophils are leukocytes associated with asthma and allergic diseases. This project explores the role of TMEM103, a novel protein, in eosinophils.

描述（申请人提供）：嗜酸性粒细胞与许多过敏性疾病相关，包括哮喘、特应性皮炎、药物反应和慢性荨麻疹。无数的嗜酸性粒细胞相关的疾病，使我们调查的发展嗜酸性粒细胞从造血干细胞，试图了解信号，促使嗜酸性粒细胞的生产在骨髓中。发展中的嗜酸性粒细胞的基因分析表明，一种新的假设蛋白质表达为mRNA。TMEM 103 mRNA在IL-5刺激的CD 34+细胞、骨髓和成熟嗜酸性粒细胞中表达。转录本TMEM 103已被证明与核糖体相关，并且该蛋白质已在发育中的嗜酸性粒细胞中通过蛋白质印迹分析鉴定。该基金建议通过产生针对该蛋白质的单克隆抗体，然后纯化和测序该蛋白质，进一步验证TMEM 103的存在。此外，将通过RNA沉默研究TMEM 103的功能，然后研究形态学和转录谱变化。公共卫生相关性：嗜酸性粒细胞是与哮喘和过敏性疾病相关的白细胞。该项目探讨了TMEM 103，一种新的蛋白质，在嗜酸性粒细胞中的作用。