EVALUATION OF PDE 5 ON ENDOTHEIAL ACTIVITY BY ACETYLCHOLINE IONTOPHORESIS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 1.膜上皮是血管系统的重要组成部分，已有文献证明它在控制心血管危险因素中起着至关重要的作用。磷酸二酯酶5(PDE 5)物质已被证明通过灭活环鸟苷磷酸(CGMP)，它们增加了内皮系统(2，3)产生的NO的利用率。 2.我们开发了一种简单的非侵入性检测(乙酰胆碱离子导入-ACH IPH)，以确定内皮系统的状态。通过将这种方法应用于正常受试者以及患有外周动脉疾病、糖尿病、高血压疾病和高胆固醇血症的患者，我们将能够确定给予上述受试者组的PDE 5药物是否会改善他们的内皮活性。在阳性病例中，这将代表患者心血管危险因素的显着改善。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. 1. The enod thelium is a vital component of the vascular system and it has been documented that it plays a vital roel in the control of cardiovascular risk factors (1). Phosphodistrerase type 5 (PDE 5)substances have been shown that by inactivating cyclic guanosine phosphates (cGMP), they increase the availability of NO produced by the endothelial sytem (2,3). 2. We have developed a simple non-invasive test ( acetylcholine ionyophoresis - ACH Iph), identifying the status of the endothelial system. BY applying this methodolgy to normal subjects as wells as to patients with peripheral arterial disease, diabetes, hypertensive disease and hypercholesterolemia, we will be able to determine if PDE 5 medication, given to the described groups of subjects, will improve their endothelial activity. In the positive case it will represent a significant improvement of patient's cardiovascualr risk factors.