siRNA Library Screening for Pharmacologic Radiation Mitigators

电离辐射诱导细胞中的RNA，这些RNA可以在辐射前被siRNA靶向以识别新的 辐射防护药物的靶标。我们已经成功地使用这一策略来识别一种常见的药物， 格列本脲作为一种新型的辐射防护剂。我们现在建议应用这一策略来确定辐射缓释剂 药物利用辐射敏感的人体细胞靶点和siRNA在照射后进行筛选。1**具体目标是 优化和部署用于检测新缓解药物的RNA靶标的哨兵细胞。第二个目标将是 使用可药物siRNA文库和小分子文库来鉴定辐射损伤缓释剂。这个 第三个目标将是确定与优先药物积极互动的目标，以促进辐射缓解 效果。

Ionizing irradiation induces RNAs in cells which can be targeted prior to irradiation by siRNA to identify novel targets for radioprotector drugs. We have successfully used this strategy to identify a co,mmon drug, Glyburide, as a novel radioprotector. We now propose to apply this strategy to identify radiation mitigator drugs using radiosensitive human cell targets and siRNA screening after irradiation. The 1** Specific Aim will optimize and deploy sentinel cells for detecting RNA targets for new mitigator drugs. The second aim will be to use druggable siRNA libraries and small molecule libraries to identify radiation damage mitigators. The third aim will be to identify targets that interact positively with prioritized drugs to advance radiation mitigation effects.