LARGE-SCALE PROTEIN AND PEPTIDE PRODUCTION CORE FACILITY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 蛋白质和多肽生产核心设施的负责人Ralph Henry教授与Robert Beitle教授合作开发了高密度补料分批培养技术，该技术每升生产的蛋白质比传统摇床培养方法多十倍以上。这种方法对于满足基于核磁共振的结构研究的蛋白质标记需求尤其关键，在这种结构研究中，使用大量的培养物来克服蛋白质表达不佳的问题，禁止使用昂贵的13C和15N同位素标记。核心设施中的设备包括一套生产设备，其中包括四个Applikon生物反应器、一个应用生物系统气相蛋白质测序仪和多肽合成器、Beckman分析和制备超速离心机、两个Akta Explorer FPLC系统和许多高效液相装置。购买核心设备所需的800 000美元几乎全部由其他赠款和机构基金提供。该大学已承诺提供机构资金，支持曾傑瑞·霍姆斯利和K.Z.Shein的长期工作人员职位，他们负责维护核心仪器。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Professor Ralph Henry, the director of the Protein and Peptide Production Core facility, has worked with Professor Robert Beitle in the development of high density fed batch culture technology, which can produce over ten times more protein per liter than traditional shaking culture methods. Such an approach is especially critical to meet protein labeling demands for NMR-based structure studies, where the use of large culture volumes to overcome 'poor' protein expression prohibits the use of expensive 13C and 15N isotopic labels. Equipment in the Core Facility includes a production suite consisting of four Applikon Bioreactors, an Applied Biosystems gas phase protein sequenator and peptide synthesizer, Beckman analytical and preparative ultracentrifuges, two AKTA Explorer FPLC systems, and numerous HPLC units. Nearly all of the $800,000 needed for the purchase of the equipment in the core has been provided by other grants and institutional funds. The University has committed institutional funds to support permanent staff positions for Jerry Homesley and K.Z. Shein, who maintain the instrumentation in the core.