GENOME-WIDE VIEW OF REPLICATION FORK PROGRESSION AND ARREST

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 真核生物中的DNA复制在萌芽酵母基因组水平上已经得到了广泛的研究。这些研究已经形成了这样一幅图景：DNA复制从大量来源开始，并在基因组的不同部分以非常不同的速度进行。在野生型酵母基因组的许多特定位置，也描述了前进的叉子停顿。这些数据被解释为复制分叉进程的动力学受到局部染色质结构或体系结构的强烈影响。在这里，我们监测了复制叉偶联GINS复合体在细胞周期中的基因组关联。描述这项工作的手稿已经出版： M.D.Sekedat，D.Feny？，R.S.Rogers，A.J.Tackett，J.D.Aitchison，B.T.Chait：Gins Motion Reveals Replication Fork Procedure is Every Uniform All the Yep Genome，Mol Systems Biol，6(2010)353 我们目前正在将这项工作扩展到研究epsilon和Delta dna聚合酶的动力学。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. DNA replication in eukaryotes has been extensively studied at the genome wide level in budding yeast. These studies have led to a picture wherein DNA replication initiates from a large number of origins and progresses at widely variable rates over different parts of the genome. The progressing fork has also been described to pause at numerous specific sites in the genome of wild type yeast. These data have been interpreted to mean that the dynamics of replication fork progression are strongly affected by local chromatin structure or architecture. Here, we monitored the genomic association of the replication fork-coupled GINS complex through the cell cycle. A manuscript describing this work has been published: M.D. Sekedat, D. Feny¿, R.S. Rogers, A.J. Tackett, J.D. Aitchison, B.T. Chait "GINS Motion Reveals Replication Fork Progression is Remarkably Uniform Throughout the Yeast Genome" Mol Systems Biol, 6 (2010) 353 We are currently extending this work to the study of the dynamics of the epsilon and delta DNA polymerases