Development of Next Generation Mass Spectrometric Instrumentation for Proteomics

开发下一代蛋白质组学质谱仪器

基本信息

  • 批准号:
    10240528
  • 负责人:
  • 金额:
    $ 70.91万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2019
  • 资助国家:
    美国
  • 起止时间:
    2019-09-01 至 2024-08-31
  • 项目状态:
    已结题

项目摘要

Project Summary/Abstract Currently, most mass spectrometric (MS) analyses for proteomics are performed in sequential mode, wherein various species in a sample are selected and interrogated one after another. As a consequence of the finite time needed to examine each species in turn, sequential mode MS suffers from inescapable limitations in sensitivity, speed and ability to analyze all ions, especially when the composition of the ion beam is complex and rapidly changing. These limitations have kept vast tracts of biology and biomedicine, including, for example, deep single cell proteome analysis, out of reach of the current MS technology. In the present proposal, we posit that sensitivity, speed and dynamic range can be vastly improved by performing MS in parallel (by analogy to Next Generation DNA Sequencing), thus overcoming the technical barriers inherent to current commercial mass spectrometers that operate largely in sequential mode. Here, we propose to develop new MS instrumentation to execute MS in a massively parallel manner, with two major objectives in mind: Objective 1. Increase the sensitivity, speed and depth of proteome analyses by up to and ultimately beyond 1000-fold. The current sequential MS approaches can be likened to sampling the Niagara Falls with a bucket, where the majority of the sample is wasted. Our proposed parallel MS technology is designed to eliminate this immense waste. Objective 2. Filter noise in real time to eliminate unwanted ion background prior to MS analysis, thereby maximizing the MS utilization of the sample ions of interest and the resulting signal-to- noise ratios, as well as providing increased dynamic range to measure very low abundance components in the presence of highly abundant components. Successful attainment of these objectives will allow deep, comprehensive, high throughput analyses of proteomes in cases where sample availability is limiting, where the components of interest elude detection due to their low abundance, or when single cell analysis is needed to address the biological or biomedical question at hand. Success in this endeavor will propel forward many areas of basic and applied biomedical research that require proteomic analyses in a manner analogous to the immense progress that has been made through development of Next Generation DNA Sequencing.
项目总结/文摘

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Brian T Chait其他文献

Brian T Chait的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Brian T Chait', 18)}}的其他基金

An Encyclopedia of the Adipose Tissue Secretome to Identify Mediators of Health and Disease
脂肪组织分泌组百科全书,用于识别健康和疾病的介质
  • 批准号:
    10907127
  • 财政年份:
    2021
  • 资助金额:
    $ 70.91万
  • 项目类别:
An Encyclopedia of the Adipose Tissue Secretome to Identify Mediators of Health and Disease
脂肪组织分泌组百科全书,用于识别健康和疾病的介质
  • 批准号:
    10670351
  • 财政年份:
    2021
  • 资助金额:
    $ 70.91万
  • 项目类别:
An Encyclopedia of the Adipose Tissue Secretome to Identify Mediators of Health and Disease
脂肪组织分泌组百科全书,用于识别健康和疾病的介质
  • 批准号:
    10295523
  • 财政年份:
    2021
  • 资助金额:
    $ 70.91万
  • 项目类别:
An Encyclopedia of the Adipose Tissue Secretome to Identify Mediators of Health and Disease
脂肪组织分泌组百科全书,用于识别健康和疾病的介质
  • 批准号:
    10445049
  • 财政年份:
    2021
  • 资助金额:
    $ 70.91万
  • 项目类别:
Development of Next Generation Mass Spectrometric Instrumentation for Proteomics
开发下一代蛋白质组学质谱仪器
  • 批准号:
    10470270
  • 财政年份:
    2019
  • 资助金额:
    $ 70.91万
  • 项目类别:
Development of Next Generation Mass Spectrometric Instrumentation for Proteomics
开发下一代蛋白质组学质谱仪器
  • 批准号:
    10707071
  • 财政年份:
    2019
  • 资助金额:
    $ 70.91万
  • 项目类别:
Development of Next Generation Mass Spectrometric Instrumentation for Proteomics
开发下一代蛋白质组学质谱仪器
  • 批准号:
    9790251
  • 财政年份:
    2019
  • 资助金额:
    $ 70.91万
  • 项目类别:
Development of Next Generation Mass Spectrometric Instrumentation for Proteomics
开发下一代蛋白质组学质谱仪器
  • 批准号:
    10005419
  • 财政年份:
    2019
  • 资助金额:
    $ 70.91万
  • 项目类别:
TR&D Project 3. The Analysis Stage II: Tools for Analyzing the Connectivity and Morphology of Macromolecular Assemblies
TR
  • 批准号:
    10621359
  • 财政年份:
    2014
  • 资助金额:
    $ 70.91万
  • 项目类别:
TR&D Project 3. The Analysis Stage II: Tools for Analyzing the Connectivity and Morphology of Macromolecular Assemblies
TR
  • 批准号:
    10401762
  • 财政年份:
    2014
  • 资助金额:
    $ 70.91万
  • 项目类别:

相似国自然基金

层出镰刀菌氮代谢调控因子AreA 介导伏马菌素 FB1 生物合成的作用机理
  • 批准号:
    2021JJ40433
  • 批准年份:
    2021
  • 资助金额:
    0.0 万元
  • 项目类别:
    省市级项目
寄主诱导梢腐病菌AreA和CYP51基因沉默增强甘蔗抗病性机制解析
  • 批准号:
    32001603
  • 批准年份:
    2020
  • 资助金额:
    24.0 万元
  • 项目类别:
    青年科学基金项目
AREA国际经济模型的移植.改进和应用
  • 批准号:
    18870435
  • 批准年份:
    1988
  • 资助金额:
    2.0 万元
  • 项目类别:
    面上项目

相似海外基金

Tribal Intertidal Digital Ecological Surveys Project: Using Large-Area Imaging to Assess Intertidal Biological Response to Changing Oceanographic Conditions in Partnership with Indigenous Nations
部落潮间带数字生态调查项目:与土著民族合作,利用大面积成像评估潮间带生物对不断变化的海洋条件的反应
  • 批准号:
    532685-2019
  • 财政年份:
    2022
  • 资助金额:
    $ 70.91万
  • 项目类别:
    Postgraduate Scholarships - Doctoral
Tribal Intertidal Digital Ecological Surveys Project: Using Large-Area Imaging to Assess Intertidal Biological Response to Changing Oceanographic Conditions in Partnership with Indigenous Nations
部落潮间带数字生态调查项目:与土著民族合作,利用大面积成像评估潮间带生物对不断变化的海洋条件的反应
  • 批准号:
    532685-2019
  • 财政年份:
    2020
  • 资助金额:
    $ 70.91万
  • 项目类别:
    Postgraduate Scholarships - Doctoral
biological interactions among forest-dwelling fungus gnats and their natural enemies in shiitake mashroom production area
香菇产区森林真菌蚊与其天敌之间的生物相互作用
  • 批准号:
    19K06152
  • 财政年份:
    2019
  • 资助金额:
    $ 70.91万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Tribal Intertidal Digital Ecological Surveys Project: Using Large-Area Imaging to Assess Intertidal Biological Response to Changing Oceanographic Conditions in Partnership with Indigenous Nations
部落潮间带数字生态调查项目:与土著民族合作,利用大面积成像评估潮间带生物对不断变化的海洋条件的反应
  • 批准号:
    532685-2019
  • 财政年份:
    2019
  • 资助金额:
    $ 70.91万
  • 项目类别:
    Postgraduate Scholarships - Doctoral
To what extent does governance play a role in how effectively a marine protected area in the Irish Sea reaches its biological and socioeconomic goals?
治理在多大程度上对爱尔兰海海洋保护区如何有效实现其生物和社会经济目标发挥作用?
  • 批准号:
    2287487
  • 财政年份:
    2019
  • 资助金额:
    $ 70.91万
  • 项目类别:
    Studentship
War and Biological Ageing in Vietnam: A Planning Grant to Foster Collaboration on a Novel Area of Global Research in Health and Ageing
越南的战争与生物衰老:一项规划拨款,以促进全球健康与老龄化研究新领域的合作
  • 批准号:
    404425
  • 财政年份:
    2019
  • 资助金额:
    $ 70.91万
  • 项目类别:
    Miscellaneous Programs
Impact assessment of Noctiluca scintillans red tide on nutrient dynamics, biological processes in lower trophic levels and material cycle in the neritic area of Sagami Bay
夜光藻赤潮对相模湾浅海区营养动态、低营养层生物过程和物质循环的影响评估
  • 批准号:
    18K05794
  • 财政年份:
    2018
  • 资助金额:
    $ 70.91万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Large-area graphene based chemical and biological sensors
基于大面积石墨烯的化学和生物传感器
  • 批准号:
    355863-2011
  • 财政年份:
    2015
  • 资助金额:
    $ 70.91万
  • 项目类别:
    Discovery Grants Program - Individual
Large-area graphene based chemical and biological sensors
基于大面积石墨烯的化学和生物传感器
  • 批准号:
    355863-2011
  • 财政年份:
    2014
  • 资助金额:
    $ 70.91万
  • 项目类别:
    Discovery Grants Program - Individual
Theoretical simulation and experimental study on biological weathering mechanism of the rock around coastal area in Yaeyama Islands
八重山群岛沿岸岩石生物风化机制的理论模拟与实验研究
  • 批准号:
    26790079
  • 财政年份:
    2014
  • 资助金额:
    $ 70.91万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了