P-30 Core Grant for Vision Research

P-30 视觉研究核心补助金

• 批准号: 8058740
• 负责人: Janey L Wiggs
• 金额: $ 47.14万
• 依托单位: MASSACHUSETTS EYE AND EAR INFIRMARY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8058740
• 关键词: Animals; Applications Grants; Award; Biological; Blindness; Blood; Cancer Center Support Grant; Cancer Grant Supplements (P30); Caring; Clinical; Clinical Research; Collaborations; Contracts; Core Facility; Cornea; DNA Sequence; DNA analysis; Diagnosis; Diagnostic; Disease; Ear; Educational process of instructing; Endowment; Environment; Equipment; Evaluation; Eye; Eye Development; Eye diseases; Facility Designs; Faculty; Foundations; Functional disorder; Funding; Genetic Research; Glaucoma; Goals; Grant; Head; Image; Immunofluorescence Immunologic; Immunology; Individual; Institution; Joints; Laboratories; Laser Scanning Confocal Microscopy; Lead; Life; Massachusetts; Medical Students; Mentored Clinical Scientist Development Program; Mentors; Methods; Molecular; Molecular Biology; Molecular Genetics; Morphology; Ocular Pathology; Ophthalmologist; Ophthalmology; Optics; Panthera leo; Participant; Patients; Pharmacologic Substance; Physicians; Physiology; Pilot Projects; Principal Investigator; Process; Program Development; Qualifying; Recruitment Activity; Research; Research Institute; Research Personnel; Research Project Grants; Research Support; Research Training; Resources; Retina; Retinal; Retinal Diseases; SECTM1 gene; Sampling; Scientist; Services; Techniques; Technology; Therapeutic; Time; Tissues; Training; Training Programs; Transmission Electron Microscopy; United States National Institutes of Health; Vision; Vision research; Work; base; fighting; in vivo; innovation; insight; light microscopy; medical schools; member; prevent; professor; programs; research study; vision science; volunteer

DESCRIPTION (provided by applicant): The proposed Core facilities are designed to enhance and make more efficient the NEI-supported research carried out at the Massachusetts Eye and Ear Infirmary. The participating investigators conduct research involving the retina, retinal diseases, glaucoma, diseases and immunology of the cornea, and ocular development. The proposed Core is organized into the following four modules. 1. The Morphology Module provides resources, services, training, and technical assistance for investigators who require laser scanning confocal microscopy, transmission electron microscopy, light microscopy, immunofluorescence analysis, and related technologies. 2. The Molecular Biology Module provides services and equipment and DNA sequencing currently required for state-of-the-art molecular biology experiments involving vision science. 3. The Clinical Interface Module provides investigators with centralized and efficient processing of blood and other types of samples derived from patients who volunteer to participate in molecular genetics research and other research of the participating investigators. As such, it serves as the interface between clinicians who diagnose and treat patients with ocular disease and the clinician-scientists and basic scientists who wish to conduct experiments that require the analysis of DNA or other biological compounds derived from those patients. 4. The in Vivo Imaging and Function Module is a new module that will use non-invasive techniques to evaluate the status of ocular tissues in living animals used in vision research. The two technologies to be initially offered by this module will be optical coherence tomographic (OCT) imaging and electroretinographic (ERG) evaluation of retinal function. These methods will allow investigators to follow the course of ocular disease over time in individual animals, thereby reducing the number of animals needed for vision research as well as providing new insights into the physiology and pathology of ocular disease. This Core will augment our institution's commitment to devote major resources to the study of ocular diseases of its patients, especially diseases that still result in blindness despite current therapies, with the long-term goal of developing new therapies for these conditions.

描述（由申请人提供）：拟议的核心设施旨在加强和提高NEI支持的研究在马萨诸塞州眼耳医院进行的效率。参与的研究人员进行涉及视网膜、视网膜疾病、青光眼、角膜疾病和免疫学以及眼部发育的研究。拟议的核心方案分为以下四个模块。1.形态学模块为需要激光扫描共聚焦显微镜、透射电子显微镜、光学显微镜、免疫荧光分析和相关技术的研究人员提供资源、服务、培训和技术援助。2.分子生物学模块提供涉及视觉科学的最先进的分子生物学实验所需的服务和设备以及DNA测序。3.临床接口模块为研究者提供血液和其他类型样本的集中和有效处理，这些样本来自自愿参与分子遗传学研究和参与研究者的其他研究的患者。因此，它是诊断和治疗眼部疾病患者的临床医生与希望进行需要分析来自这些患者的DNA或其他生物化合物的实验的临床科学家和基础科学家之间的接口。4.体内成像和功能模块是一个新的模块，将使用非侵入性技术来评估视觉研究中使用的活体动物的眼组织状态。该模块最初提供的两项技术将是光学相干断层扫描（OCT）成像和视网膜电图（ERG）视网膜功能评估。这些方法将使研究人员能够在个体动物中随时间推移跟踪眼部疾病的过程，从而减少视觉研究所需的动物数量，并为眼部疾病的生理学和病理学提供新的见解。这一核心将加强我们机构的承诺，将主要资源投入到患者的眼部疾病研究中，特别是尽管有目前的治疗方法，但仍然导致失明的疾病，长期目标是为这些疾病开发新的治疗方法。