Adult Stem Cell Enrichment via Biomechanical Characterization

脂肪基质/干细胞(ASCs)为生物医学领域提供了令人兴奋的机会 工程学。ASCs作为组织再生过程中的细胞来源很有吸引力，因为它可以 可获得性和有限的供体部位发病率。过去的研究表明，这些细胞可以分化 使用培养液补充剂转化为各种类型的细胞，有效地引导它们走向特定的 兴趣：软骨细胞、成骨细胞和脂肪细胞。我们实验室目前的研究充分表明 已分化细胞和未分化ASCs具有其细胞特有的生物力学特性 类型(即软骨细胞、成骨细胞、脂肪细胞)。该奖项的独立阶段的目标是 根据生物力学性质在吸脂物中分离细胞以产生谱系特异性细胞来源 这将提高工程组织的功能性能。我们假设工程设计了 含有较高比例的组织特异性干细胞的构建物具有更好的生物力学性能 和生化特性，而不是播种了更加异质的收获群体的构建。为了测试 为此，我们将根据细胞的生物力学特性对吸脂废物组织中的细胞进行分类，并随后 评估他们的多才多艺。在这些研究的同时，我们将设计和制造一种装置 能够根据细胞的机械特性对大量细胞进行分选。由此产生的亚群 将具有可测量的力学性能分布，其假设为 提示ASCs具有向下分化不同间充质系的能力。组织工程软骨 然后，将使用异质和机械分类的细胞群体来生长构建物 确定干细胞的增多如何影响组织形成的质量。整个拟议的项目将 在机械转导、干细胞生物学和组织领域取得重大进展 工程学。

Adipose-derived stromal/stem cells (ASCs) pose exciting opportunities for the field of biomedical engineering. ASCs are attractive as a cell source for tissue regeneration procedures because of their ready availability and limited donor-site morbidity. Past research has shown that these cells can be differentiated into a variety of cell types using media supplements, effectively guiding them towards specific lineages of interest; chondrocytic, osteoblastic, and adipocytic. Current studies in our laboratory indicate that fully differentiated cells and undifferentiated ASCs possess biomechanical properties characteristic of their cell type (i.e. chondrocyte, osteoblast, adipocyte). The goal of the Independent Phase of this Award is to separate ceiis within lipoaspirate by their biomechanical properties to produce lineage-specific cell sources that will improve the functional performance of engineered tissues. We hypothesize that engineered constructs containing a higher percentage of tissue-specific stem ceils wili possess better biomechanical and biochemical properties than constructs seeded with a more heterogeneous harvest population. To test this, we will sort ceils from liposuction waste tissue by their biomechanical properties and subsequently evaluate their multipotent character. Concurrent with these studies, we will design and fabricate a device capable of sorting large numbers of cells based on their mechanical characteristics. The resulting subpopulations will possess measurable distributions of mechanical properties, which are hypothesized to indicate an ASCs ability to differentiate down different mesenchymal lineages. Tissue engineered cartilage constructs will then be grown using either heterogeneous and mechanicaiiy-sorted cell populations to determine how stem ceil enrichment affects the quality of tissue formation. The entire proposed project will produce significant advances in the areas of mechanotransduction, stem cell biology, and tissue engineering.