斑马鱼 K99 到 R00 过渡神经嵴的迁移和生存-猫眼课题宝

斑马鱼 K99 到 R00 过渡神经嵴的迁移和生存

批准号：

8099548

负责人：

Rodney A. Stewart

金额：

$24.9万

依托单位：

UNIVERSITY OF UTAH

依托单位国家：

美国

项目类别：

财政年份：

2009

资助国家：

美国

项目状态：

已结题

起止时间：

2009-07-15 至 2013-06-30

来源：

https://reporter.nih.gov/project-details/8099548

关键词：

Address Animals Apoptosis Appointment Behavior Biochemistry Blood Circulation Blood capillaries Cell Adhesion Molecules Cell Death Cell Survival Cell-Cell Adhesion Cells Data Defect Development Distant Dorsal E-Cadherin Embryo Endothelium Environment Epithelial Event Faculty Family Family member Gene Expression Genes Genetic Genetic Epistasis Glioma Goals Human Genome Huntsman Cancer Institute at the University of Utah Institutes Knowledge Laboratories Link Malignant Neoplasms Mediating Mediator of activation protein Mesenchymal Modeling Molecular Mus Mutation Neoplasm Metastasis Neural Crest Neural Crest Cell Neural tube Neurobiology Neuroblastoma Oncogenic Organ Orthologous Gene Pathway interactions Pattern Peripheral Nerve Sheath Neoplasm Phase Phenocopy Phenotype Process Proliferating Property Proteins Puma RNA Research Research Personnel Research Proposals Role Science Signal Transduction Site Snails Staging Stem cells Synteny System Testing Tissues Training Transcription factor genes Tumor-Derived Zebrafish base cadherin-6 cancer cell capillary career cell motility design in vivo melanoma member migration mutant neoplastic cell novel null mutation oncology overexpression progenitor slug tool transcription factor tumor

项目摘要

项目成果

Rodney A. Stewart的其他基金

相似基金

相关文献

中文摘要

翻译

点击翻译按钮获取中文摘要

英文摘要

The goal oflhis application is to use the zebrafish as a |nodel to dissect the molecular connponents of Foxd3-mediated neural crest (NC) cell survival and migration end to determine if these pathways promote metastasis in NG-derived tumors in vivo. The zebrafish with its close synteny to the human genome and its conserved molecular pathways regulating the development of tissues and organs, Offers a powerful tool with which to conduct such research. We previoulsy identified and characterized a zebrafish foxd3 mutant line that has specific NC ceil survival and migration defects. We also identified the Snai1b transcription factor-as a critical mediator of Foxd3 function. The underlying hypothesis of this applicatipn is that knowledge of the Foxd3 pathway wiII provide an understanding environments, requirement for the metaststasis of NC-derived tumors. In Aim 1, genetic epistasis and biochemistry will determine if Foxd3 directly activates snai1b expression. This aim will also will also determine whether Snai 1b inhibits NC apoptosis by repressing the expression of one or more BH3-only genes, and promotes NC migration by repressing cadherin-6 expression . In Aim 2, a list of genes identified in a microarray screen during the bK99 phase will abe analyzed for thier NC expression pattern and knockdown phenotypes, as well as their ability to rescue NC defects in foxd3 mutant and snai 1b MO-injected embryos. In Aim 3, foxd3 and snai1b will be expressed in developing NC cells to examine their potential roles in promoting metastasis in established zebrafish NC tumors, including a new model identified during the K99 phase. Rodney Stewart will continue to receive advanced training in genetics, neurobiology and oncology throughout his career. This research proposal is designed to support the candidate as an independent investigator during the first 3 years of a faculty appointment in the Department of Oncology Sciences at the Huntsman Cancer Institute at the University of Utah. Huntsrnan Cancer Institute at the yniyersity of-Utahnd

期刊论文(0)

专著(0)

科研奖励(0)

会议论文