WNPRC GENETICS SERVICES UNIT

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 目的：为核心、校园和非宿主研究者提供复杂和专业的遗传学资源和专业知识。 进展和关切 2008年，遗传学服务中心专注于改善猕猴MHC基因分型的可及性。基于微卫星的单倍型分型现在用于样本认证，谱系验证，以及非马达加斯加食蟹猴的基因分型。专业的MHC基因分型服务也可用于印度和中国起源的恒河猴，猪尾猕猴，食蟹猴从不同的地理来源。Genetics Services正在率先开发高分辨率，灵敏度和经济的检测方法，用于猕猴的全面MHC基因分型。2008年期间，这包括为外部研究者引入一项积极的收费服务计划，即使用桑格和罗氏/454下一代平台进行高通量、基于序列的MHC I类基因分型。 资源访问的分配 2008年，遗传服务处向20多个实验室提供了MHC基因分型支持。 此外，我们与NCRR灵长类中心遗传学和基因组学工作组以及基因组库工作组合作，该工作组已经从各种非人类灵长类动物中产生了大量的基因组DNA样本库。 传播 奥康纳博士、遗传学服务PI和Wiseman博士就NHP遗传学发表了多篇演讲并撰写了七篇出版物（见下文）。我们要求使用遗传学服务的项目在手稿和演示文稿中承认该服务。 培训 在奥康纳博士和他的工作人员参加的科学会议上，经常讨论MHC基因分型的方法。2008年，这包括为国际艾滋病疫苗倡议（IAVI）和在NIH为NIAID，DIADS和疫苗研究计划进行的多次演讲。 此外，还在以下科学会议上发表了演讲：第三届灵长类基因组学和人类疾病国际会议、454生命科学用户组会议、分子病理学协会2008年年会、NIH移植工作组NHP测定讲习班、利用非人类灵长类动物艾滋病模型中的系统生物学卫星会议、第26届非人类灵长类动物艾滋病模型年度研讨会。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To provide core, campus and non-host investigators with sophisticated and specialized genetics resources and expertise. Progress and concerns In 2008 Genetics Services focused on improving access to MHC genotyping in macaques. Microsatellite-based haplotyping is now used for sample authentication, pedigree verification, and genotyping of Mauritian-origin as well as non-Mauritian cynomolgus macaques. Specialized MHC genotyping services are also available for Indian- and Chinese-origin rhesus macaques, pig-tailed macaques, and cynomolgus macaques from various geographic origins. Genetics Services is taking the lead in developing high-resolution, sensitive, and economical assays for comprehensive MHC genotyping in macaques. During 2008, this included the introduction of an active fee-for-service program of high throughput, sequence-based MHC class I genotyping using both Sanger and Roche/454 next-generation platforms for external investigators. Allocation of resource access The Genetics Service provided MHC genotyping support to more than 20 laboratories in 2008. In addition, we worked with the NCRR Primate Centers Working Group on Genetics and Genomics, as well as the Genome Banking Working Group that has generated a large repository of genomic DNA samples from a wide variety of nonhuman primates. Dissemination Dr. O'Connor, the PI for Genetics Services, and Dr. Wiseman presented multiple lectures and authored seven publications on NHP genetics (see below). We request that projects utilizing Genetics Service acknowledge the service in manuscripts and presentations. Training Methods for MHC genotyping are regularly discussed during scientific conferences attended by Dr. O'Connor and his staff. In 2008, this included multiple presentations for the International AIDS Vaccine Initiative (IAVI) and at the NIH for NIAID, DIADS & the Vaccine Research Program. In addition, lectures were given at the following scientific meetings: 3rd International Conference on Primate Genomics and Human Disease, 454 Life Sciences User Group Meeting, Association for Molecular Pathology 2008 Annual Meeting, NIH Transplantation Working Group NHP Assays Workshop, Exploiting Systems Biology in Nonhuman Primate Models for AIDS Satellite Meeting, 26th Annual Symposium for Nonhuman Primate Models for AIDS.