WNPRC GENETICS SERVICES UNIT

WNPRC 遗传学服务单位

• 批准号: 7958778
• 负责人: David H. O'Connor
• 金额: $ 39.32万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7958778
• 关键词: AIDS Vaccines; Acquired Immunodeficiency Syndrome; Base Sequence; Biological Assay; Biological Sciences; Chinese People; Computer Retrieval of Information on Scientific Projects Database; DNA; Educational workshop; Family suidae; Fee-for-Service Plans; Funding; Genetic; Genetic Services; Genome; Genomics; Genotype; Grant; Group Meetings; Improve Access; Institution; International; International AIDS; Laboratories; Lead; MHC Class I Genes; Macaca; Macaca mulatta; Manuscripts; Methods; Microsatellite Repeats; Modeling; National Center for Research Resources; National Institute of Allergy and Infectious Disease; Primates; Publications; Research; Research Personnel; Resolution; Resource Allocation; Resources; Sampling; Services; Source; Systems Biology; Tail; Training; Transplantation; United States National Institutes of Health; Vaccine Research; Wisconsin; Work; base; genetic pedigree; genetic resource; human disease; lectures; meetings; molecular pathology; next generation; nonhuman primate; programs; repository; symposium; working group

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To provide core, campus and non-host investigators with sophisticated and specialized genetics resources and expertise. Progress and concerns In 2008 Genetics Services focused on improving access to MHC genotyping in macaques. Microsatellite-based haplotyping is now used for sample authentication, pedigree verification, and genotyping of Mauritian-origin as well as non-Mauritian cynomolgus macaques. Specialized MHC genotyping services are also available for Indian- and Chinese-origin rhesus macaques, pig-tailed macaques, and cynomolgus macaques from various geographic origins. Genetics Services is taking the lead in developing high-resolution, sensitive, and economical assays for comprehensive MHC genotyping in macaques. During 2008, this included the introduction of an active fee-for-service program of high throughput, sequence-based MHC class I genotyping using both Sanger and Roche/454 next-generation platforms for external investigators. Allocation of resource access The Genetics Service provided MHC genotyping support to more than 20 laboratories in 2008. In addition, we worked with the NCRR Primate Centers Working Group on Genetics and Genomics, as well as the Genome Banking Working Group that has generated a large repository of genomic DNA samples from a wide variety of nonhuman primates. Dissemination Dr. O'Connor, the PI for Genetics Services, and Dr. Wiseman presented multiple lectures and authored seven publications on NHP genetics (see below). We request that projects utilizing Genetics Service acknowledge the service in manuscripts and presentations. Training Methods for MHC genotyping are regularly discussed during scientific conferences attended by Dr. O'Connor and his staff. In 2008, this included multiple presentations for the International AIDS Vaccine Initiative (IAVI) and at the NIH for NIAID, DIADS & the Vaccine Research Program. In addition, lectures were given at the following scientific meetings: 3rd International Conference on Primate Genomics and Human Disease, 454 Life Sciences User Group Meeting, Association for Molecular Pathology 2008 Annual Meeting, NIH Transplantation Working Group NHP Assays Workshop, Exploiting Systems Biology in Nonhuman Primate Models for AIDS Satellite Meeting, 26th Annual Symposium for Nonhuman Primate Models for AIDS.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 目的：为核心研究人员、校园研究人员和非东道主研究人员提供成熟和专业的遗传学资源和专业知识。 进展和关切 2008年，遗传学服务的重点是改善猕猴MHC基因分型的可及性。基于微卫星的单倍体分析现在被用于样品鉴定、系谱核实和毛里求斯原产猕猴和非毛里求斯食蟹猴的基因分型。专门的MHC基因分型服务也适用于印度和中国起源的恒河猴、猪尾猕猴和来自不同地理来源的食蟹猴。遗传学服务公司正在率先开发高分辨率、灵敏和经济的猕猴MHC基因分型综合检测方法。2008年期间，引入了一项积极的收费服务计划，利用Sanger和Roche/454下一代平台进行外部研究人员的高通量、基于序列的MHC I类基因分型。 资源访问权限的分配 2008年，遗传局为20多个实验室提供了MHC基因分型支持。此外，我们与NCRR灵长类中心遗传学和基因组学工作组以及基因组库工作组合作，该工作组已经从各种非人类灵长类动物中产生了大量基因组DNA样本。 传播 O‘Connor博士、遗传学服务PI和Wiseman博士在NHP遗传学方面发表了多场演讲并撰写了七份出版物(见下文)。我们要求利用遗传学服务的项目在手稿和演示文稿中承认这项服务。 培训 在奥康纳博士和他的工作人员参加的科学会议上，MHC基因分型的方法经常被讨论。2008年，包括在国际艾滋病疫苗倡议(IAVI)和美国国立卫生研究院为NIAID、DIADS和疫苗研究计划所做的多次演讲。此外，还在以下科学会议上发表了演讲：第三届灵长类基因组与人类疾病国际会议、454生命科学用户小组会议、分子病理学协会2008年年会、国家卫生研究院移植工作组NHP分析研讨会、为艾滋病卫星会议开发系统生物学在非人灵长类模型中的应用、第26届艾滋病非人灵长类模型年度研讨会。