MECHANISMS OF ERYTHROCYTIC INFECTIONS AND ANEMIA: NHP MODEL MALARIAL ANEMIA

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目的总体目标是详细了解疟原虫感染红细胞并诱导哺乳动物宿主贫血的分子和细胞机制。本项目的主要目的是建立非人灵长类动物贫血模型。 为了实现这些目标，一组具有血液学，寄生虫学，细胞生物学，生物化学，分子生物学，免疫学，寄生虫遗传学，功能基因组学，啮齿动物和非人灵长类动物模型专业知识的研究人员聚集在一起，共同努力。在这些研究中收集的信息有望导致疟疾感染和贫血的治疗策略的发展。到目前为止，我们已经建立和完善了一个动物模型，研究恶性疟原虫疟疾贫血的生理学使用恒河猴（Macaca mulatta）/P. coatneyi模型系统;这是一个理想的模型，检查感染的血液和骨髓样品。 在过去的一年里，通过完善的方案，在一组新的动物中建立了Coatneyi感染。感染不仅引起疟疾贫血，而且也是以弥散性血管内凝血为特征的严重疟疾的原因。与这些感染有关的手稿正在准备中，并且正在计划建立食蟹猴疟原虫-恒河猴模型以研究间日疟原虫疟疾贫血。 这种旨在研究疟疾性贫血的体内模型为理解严重疟疾疾病的多因素原因提供了相关见解。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The overall objective of this program project is to develop a detailed understanding of the molecular and cellular mechanisms by which malaria parasites infect erythrocytes and induce anemia in their mammalian hosts. The main objective of this project is to establish non-human primate models of anemia. To achieve these objectives a group of investigators with expertise in hematology, parasitology, cell biology, biochemistry, molecular biology, immunology, parasite genetics, functional genomics, rodent and non-human primate models have come together to mount a concerted effort. The information gathered in these studies is expected to lead to the development of therapeutic strategies against malarial infection and anemia. To date, we have established and refined an animal model to study the physiology of P. falciparum malarial anemia using the rhesus monkey (Macaca mulatta) / P. coatneyi model system; this is an ideal model to examine infected blood and bone marrow samples. This past year, P. coatneyi infections were established in a new group of animals with refined protocols. Infection not only caused malaria anemia but it also was the cause of severe malaria hallmarked by disseminated intravascular coagulopathy. A manuscript relating to these infections is in preparation and planning is underway to establish the P. cynomolgi - rhesus model to study P. vivax malaria anemia. This in vivo model, designed to study malarial anemia, is providing insights relevant to understanding the multi-factorial causes of severe malaria disease.