T LYMPHOCYTE, T CELL RECEPTOR, CELLULAR IMMUNITY, IMMUNOTHERAPY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本研究的目的是研究持续的急性和慢性感染以及接触肿瘤如何影响幼稚旁观者T细胞群。 在本报告所述期间，我们继续研究分析急性和慢性感染期间幼稚T细胞的状态、动力学和稳定性，并确定持续感染或持续肿瘤期间慢性抗原暴露如何影响旁观者幼稚T细胞产生免疫应答的能力。 我们还致力于确定在慢性感染或肿瘤模型期间引入治疗性细胞因子对旁观者幼稚T细胞的影响。 使用TCR转基因模型，我们通过过继转移将OVA TCR幼稚T细胞暴露于急性LCMV感染或刘易斯肺癌肿瘤移植模型，回收的OVA幼稚细胞然后确定它们响应OVA感染的能力。 我们将继续努力开发治疗时机的线索，这将导致治疗发展，以针对慢性疾病和免疫抑制期间的幼稚T细胞活力，例如艾滋病毒感染。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study is to examine how ongoing acute and chronic infections and exposure to tumors affect na¿ve bystander T cell populations. During the reporting period, we continued our studies analyzing the status, kinetics, and stability of na¿ve T cells during acute and chronic infections and to determine how chronic antigen exposure during persistent infections or persisting tumors has on the ability of bystander na¿ve T cells to mount immune responses. We also worked to determine the effect of introducing therapeutic cytokines during a chronic infection or tumor model has on bystander na¿ve T cells. Using a TCR transgenic model we exposed, by adoptive transfer, OVA TCR na¿ve T cells to acute LCMV infection or Lewis Lung Carcinoma tumor transplant model, recovered OVA na¿ve cells then determine their ability to respond to OVA infection. We will continue to work to develop clues in the timing of therapies that will lead to therapeutic developments for targeting na¿ve T cell viability during chronic diseases and immune suppression, for example HIV infection.