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DNA Extraction Service

DNA Extraction Service
DNA提取服务
批准号:
7969956
负责人:
David Winkler
金额:
$25.39万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:

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中文摘要
翻译
筛选小鼠DNA是评估在我们的菌落中出生的小鼠的关键的第一步。利用DNA所决定的遗传特性,继续进行育种选择,选择实验用动物,保证近交系小鼠遗传完整性的维持。尾部样品用蛋白水解酶消化,DNA用2 Autogen 750 DNA分离机器人分离。08财年(至9月10日)已处理48,521个样品。这是一个高度自动化的过程,非常具有成本效益。消耗性成本/尾巴约为0.45美元。对BioQual合同的监督包括与合同员工的日常对话,并在适当的时候进行访问。在过去的一年里,合同费用一直在预算范围内。
英文摘要
The screening of mouse DNA is a critical first step in evaluating mice born in our colonies. The genetic characteristics determined by the DNA is used to continue selection for breeding, to choose animals to utilize in experiments, and to assure the maintenance of genetic integrity of inbred strains of mice. Tail samples are digested using a proteolytic enzyme and the DNA is isolated utilizing 2 Autogen 750 DNA isolation robots. During FY08 (thru 10 Sept) 48,521 samples have been processed. This is a highly automated process and is very cost effective. Expendable costs/tail are approximately $0.45. Oversight of the BioQual contract consists of daily conversations with contract staff and visits when appropriate. Contract expenses have been within budget limits during the past year.
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