DNA Sequencing

DNA测序

• 批准号: 7969880
• 负责人: Steven Akiyama
• 金额: $ 80.52万
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7969880
• 关键词: Architecture; Cell Adhesion; Cell Nucleus; Cells; Chromosomes; Communities; Custom; DNA; DNA Repair; DNA Sequence; DNA Sequencing Facility; Data; Detection; Disease; Excision Repair; Family; Gene Cluster; Gene Family; Genes; Genome; Genomics; Germ Lines; Individual; Label; Link; Malignant Neoplasms; Malignant neoplasm of brain; Malignant neoplasm of liver; Malignant neoplasm of prostate; Mutagenesis; National Institute of Environmental Health Sciences; Neoplasm Metastasis; Predisposition; Research Personnel; Services; Variant; Work; cell motility; design; design and construction; genetic linkage; hormone related cancer; malignant breast neoplasm; prostate carcinogenesis; sperm protein

The DNA Sequencing Core Lab provides a variety of services to the NIEHS scientific community. The Sequencing Core assist labs in SNP discovery, mutagenesis detection, custom primer design, expression construct design, and optimization of PCR. Our main focus has been to provide DNA sequence data to individual investigators in a timely manner. The DNA Sequencing Core works with individual labs to make DNA expression constructs. In the past year we have made DNA expression constructs involved in cell migration, cell adhesion, cancer metastasis, cancer related hormones, DNA repair excision repair mechanisms and for the fluorescent labeling of cells. The various constructs were used to study a host of cancers including but not limited to breast cancer, prostrate cancer, liver cancer, and brain cancer. The Sequencing Core has also collaborates with investigators at NCI to further understand the SPANX gene family. Genetic linkage studies indicate that germ line variations in a gene or genes on chromosome Xq27-28 are implicated in prostate carcinogenesis. The linkage peak of prostate cancer overlies a region of approximately 750 kb containing five SPANX genes (SPANX-A1, -A2, -B, -C, and -D) encoding sperm proteins associated with the nucleus; their expression was also detected in a variety of cancers. We continue to further characterize this region of the genome. We speculate that the predisposition to prostate cancer in X-linked families is an example of a genomic disease caused by a specific architecture of the SPANX gene cluster.

DNA测序核心实验室为NIEHS科学界提供各种服务。测序核心协助实验室进行SNP发现、诱变检测、定制引物设计、表达构建体设计和PCR优化。我们的主要重点是及时向个别调查人员提供DNA序列数据。 DNA测序核心与各个实验室合作，制造DNA表达构建体。在过去的一年中，我们已经使DNA表达结构参与细胞迁移，细胞粘附，癌症转移，癌症相关激素，DNA修复切除修复机制和细胞的荧光标记。各种构建体用于研究许多癌症，包括但不限于乳腺癌、前列腺癌、肝癌和脑癌。测序核心还与NCI的研究人员合作，以进一步了解SPAN基因家族。遗传连锁研究表明，染色体Xq 27 -28上的一个或多个基因的种系变异与前列腺癌发生有关。前列腺癌的连锁峰覆盖约750 kb的区域，该区域包含5个编码与细胞核相关的精子蛋白的SPAN X基因（SPAN X-A1、-A2、-B、-C和-D）;在多种癌症中也检测到它们的表达。我们将继续进一步描述基因组的这一区域。我们推测X连锁家族中前列腺癌的易感性是由SPAN基因簇的特定结构引起的基因组疾病的一个例子。