Rapid Allergenic Particle Identification (RAPID)

描述（由申请人提供）： 拟议的努力结合了空气采样设备和高度集成的蛋白质微阵列，以允许以时间有效的方式估计多种蛋白质过敏原暴露。便携式空气采样装置，适合在鼻孔内，并已用于澳大利亚和欧洲的哮喘研究将用于收集样本，在一个正在进行的研究，内城纽约家庭的儿童（n=200），发展过敏和哮喘的风险增加。高度多重蛋白质微阵列，允许许多过敏原的平行评估，集成到有源互补金属氧化物半导体（CMOS）基板将被采用。CMOS的使用允许开发高度集成和敏感的设备，因为检测器直接接近传感电子器件。作为微电子学的商品技术，这种有源衬底可以以非常低的成本生产。一个建立的基于荧光的传感器基板将用于初步研究，通过简单的微流体输送增强。生物传感器技术的发展将包括努力实现实时测量能力，包括更复杂的微流体输送（包括压力激活阀），通过寿命敏感的FRET进行实时荧光传感，以及CMOS集成的基于质量的生物传感器。用于评估空气中过敏原的相同原则可应用于评估细菌和真菌病原体及其组成毒素。这些研究对公共卫生的急性和慢性措施具有深远的影响。

DESCRIPTION (provided by applicant): The proposed effort combines air sampling devices and highly-integrated protein microarrays to permit estimation of multiple protein allergen exposures in a time efficient manner. Portable air sampling devices that fit inside the nostrils and have been used in Australian and European asthma studies will be used for collection of samples in an ongoing study of inner-city New York homes of children (n=200) with increased risk for developing allergy and asthma. Highly multiplexed protein microarrays, allowing parallel assessment of many allergens, integrated onto active complementary metal-oxide-semiconductor (CMOS) substrates will be employed. The use of CMOS allows highly integrated and sensitive devices to be developed because of the immediate proximity of the detector to the sensing electronics. As the commodity technology for microelectronics, such active substrates can be produced at very low cost. An established fluorescence based sensor substrate will be employed for initial studies, augmented by simple microfluidic delivery. Biosensor technology development will include efforts toward real-time measurement capabilities including more complex microfluidic delivery (including pressure activated valves), real-time fluorescence sensing through lifetime-sensitive FRET, and CMOS-integrated mass-based biosensors. The same principles for assessment of allergens in air can be applied to assessment of bacterial and fungal pathogens and their constitutive toxins. These studies have far-reaching implications for acute and chronic measures of public health.