Rapid Allergenic Particle Identification (RAPID)

描述(由申请人提供)： 这项拟议的工作结合了空气采样设备和高度集成的蛋白质微阵列，从而能够以一种省时的方式估计多种蛋白质过敏原暴露。澳大利亚和欧洲的哮喘研究中已经使用了适合鼻孔的便携式空气采样设备，将在一项正在进行的纽约市中心儿童家庭(n=200)患过敏和哮喘风险增加的研究中收集样本。将采用高度多元化的蛋白质微阵列，允许对许多过敏原进行并行评估，并集成到活跃的互补金属氧化物半导体(CMOS)衬底上。由于探测器与传感电子设备非常接近，因此可以开发出高度集成和灵敏的器件。作为微电子的商品化技术，这种活性基板可以以非常低的成本生产。已建立的基于荧光的传感器衬底将用于初步研究，并通过简单的微流控传递进行增强。生物传感器技术的发展将包括向实时测量能力的努力，包括更复杂的微流控输送(包括压力激活阀)、通过寿命敏感的FRET进行实时荧光传感，以及集成CMOS的基于质量的生物传感器。空气中变应原评估的相同原则也可应用于细菌和真菌病原体及其构成毒素的评估。这些研究对公共卫生的急性和慢性措施具有深远的影响。

DESCRIPTION (provided by applicant): The proposed effort combines air sampling devices and highly-integrated protein microarrays to permit estimation of multiple protein allergen exposures in a time efficient manner. Portable air sampling devices that fit inside the nostrils and have been used in Australian and European asthma studies will be used for collection of samples in an ongoing study of inner-city New York homes of children (n=200) with increased risk for developing allergy and asthma. Highly multiplexed protein microarrays, allowing parallel assessment of many allergens, integrated onto active complementary metal-oxide-semiconductor (CMOS) substrates will be employed. The use of CMOS allows highly integrated and sensitive devices to be developed because of the immediate proximity of the detector to the sensing electronics. As the commodity technology for microelectronics, such active substrates can be produced at very low cost. An established fluorescence based sensor substrate will be employed for initial studies, augmented by simple microfluidic delivery. Biosensor technology development will include efforts toward real-time measurement capabilities including more complex microfluidic delivery (including pressure activated valves), real-time fluorescence sensing through lifetime-sensitive FRET, and CMOS-integrated mass-based biosensors. The same principles for assessment of allergens in air can be applied to assessment of bacterial and fungal pathogens and their constitutive toxins. These studies have far-reaching implications for acute and chronic measures of public health.