Genomics Core Facility

基因组学核心设施

• 批准号: 8340333
• 负责人: MICHAEL D TOPAL
• 金额: $ 24.5万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-23 至 2015-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8340333
• 关键词: Accounting; Animal Model; Archives; Bioinformatics; Breast; Cancer Center; Cancer Center Support Grant; Cancer and Leukemia Group B; Cell Line; Colon; Complex; Computer software; Core Facility; Custom; DNA; DNA Sequence; DNA Sequencing Facility; Data; Data Analyses; Data Set; Databases; Development; Ensure; Equipment; Experimental Designs; Funding; Future; Gene Expression; Gene Expression Alteration; Gene Expression Profiling; Genes; Genome; Genomics; Genotype; Goals; Grant; Head and Neck Neoplasms; Housing; Human; Hybridization Array; Image Analysis; Kidney; Label; Libraries; Malignant Neoplasms; Malignant neoplasm of pancreas; Maps; Measurement; Messenger RNA; Methods; Methylation; MicroRNAs; Microarray Analysis; Mus; Neurosciences; Online Systems; Pathway Analysis; Pathway interactions; Peer Review; Phase; Preparation; Price; Prostate; Quality Control; RNA; RNA Interference; RNA analysis; Regulatory Pathway; Research; Research Personnel; Research Support; Sampling; Screening procedure; Series; Services; Signal Transduction; Site; Specialist; Techniques; Technology; Testing; The Cancer Genome Atlas; Therapeutic; Walking; Work; Yeasts; anticancer research; cDNA Library; cancer gene expression; comparative genomic hybridization; cost; cost effective; functional genomics; gene interaction; genetic regulatory protein; genome-wide; high throughput screening; member; molecular pathology; next generation; programs; rectal; research study; response; tool; tumor

Genomics Core Facility The goal of the Genomics Core Facility (GCF) is to make genomic analysis products and services widely available to UNC LCCC members at cost-effective prices. Services include: producing low-cost custom microarrays for model organisms; analysis of RNA quality; support of the use of Agilent and Affymetrix gene expression microarrays and aCGH and microRNA microarrays; RNAi screening services; assisting with experimental design and analyzing results; archiving of both Agilent and Affimetrix array data; providing web based pathway analysis software; and providing "walk-up" quantitative PCR analysis. The Core adds value to the Center by making accessible complex and expensive DNA technologies to UNC LCCC members. Well-integrated genomics and bioinformatics groups interact with the microarray user to ensure robust data that is archived appropriately for future use. Highlights of research supported by the Core include: Jen Jen Yeh's profiling studies of gene expression in pancreatic cancers, Charles Perou's profiling of cancer gene expression and the effects of cancer therapeutics, and characterization of gene expression in a wide range of tumor types in support of the Cancer Genome Atlas (TCGA) Project. This year and next will see major changes. The Bioinformatics group, while remaining fully integrated, functionally is evolving into a separate core with multiple functions. The Genomics Core is also expanding to offer new techniques, including mapping of methylation sites, preparation of cDNA libraries from mRNA for mRNA-seq by the NexGen sequencing core; continuing development of an RNAi High-throughput Screening facility for functional genomics studies; and offering Nanostring technology for multiplex quantitative measurement of mRNA without amplification. Lastly, the Agilent and Affimetrix microarray components will be physically consolidated in newly renovated space with the DNA Sequencing, Mammalian Genotyping, and High Throughput (Next-Generation) Sequencing Facility. This will enable the cores to more fully integrate and flexibly service users. In 2009, the core was used by 91 investigators. Peer-review funded members accounted for 87% of total core use. Fifty-five Cancer Center members from eight programs used the core. CCSG funding of $167,411 is requested for 2010, representing 10% of the projected operating costs.

基因组学核心设施 基因组学核心设施（GCF）的目标是使基因组分析产品和服务以具有成本效益的价格广泛提供给欧洲联盟LCCC成员。服务包括：为模式生物生产低成本的定制微阵列; RNA质量分析;支持使用Agilent和Affyssin基因表达微阵列以及aCGH和microRNA微阵列; RNAi筛选服务;协助 实验设计和分析结果; Agilent和Affiliate阵列数据的存档;提供基于网络的途径分析软件;以及提供“walk-up”定量PCR分析。核心通过向CCLCC成员提供可访问的复杂和昂贵的DNA技术，为中心增加了价值。 整合良好的基因组学和生物信息学小组与微阵列用户互动，以确保适当存档以供未来使用的强大数据。核心支持的研究重点包括：Jen Jen Yeh的胰腺癌基因表达谱研究，Charles Perou的癌症基因表达谱和癌症治疗效果，以及广泛的基因表达特征。 癌症基因组图谱计划（Cancer Genome Atlas，TCGA） 今年和明年将有重大变化。生物信息学组，虽然保持完全整合，功能上正在演变成一个独立的核心，具有多种功能。Genomics Core也在不断扩展，以提供新技术，包括甲基化位点定位，通过NexGen测序核心从mRNA制备cDNA文库用于mRNA-seq;继续开发RNAi高通量筛选 用于功能基因组学研究的设施;并提供Nanostring技术，用于在不扩增的情况下对mRNA进行多重定量测量。最后，Agilent和Affiectin的微阵列组件将在新装修的空间与DNA测序、哺乳动物基因分型和高通量（下一代）测序设施进行物理整合。这将使核心能够更充分地集成和灵活地服务用户。 2009年，91名调查员使用了该核心数据库。同行评审资助的成员占核心资源使用总量的87%。来自8个项目的55名癌症中心成员使用了该核心。2010年，CCSG申请资金167 411美元，占预计业务费用的10%。