Neuroscience Core

神经科学核心

• 批准号: 8743406
• 负责人: MICHAEL Van Doren JOHNSTON
• 金额: $ 2.76万
• 依托单位: HUGO W. MOSER RES INST KENNEDY KRIEGER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8743406
• 关键词: Adrenoleukodystrophy; Amino Acid Receptor Binding; Autistic Disorder; Biochemical; Biological Assay; Brain; Cerebral Palsy; Chairperson; Developmental Disabilities; Disease; Down Syndrome; Enzymes; Equipment; Experimental Models; Fatty Acids; Gas-Liquid Chromatography; Glutamates; Head; High Pressure Liquid Chromatography; Histology; Hospitals; Imaging Techniques; Inborn Errors of Metabolism; Institutes; Leadership; Left; Lipid Biochemistry; Lipids; Mass Fragmentography; Mental Retardation; Mental Retardation and Developmental Disabilities Research Centers; Microscope; Neurosciences; Neurosciences Research; Neurotransmitter Receptor; Neurotransmitters; Peroxisomal Disorders; Psychiatry; Recruitment Activity; Refsum Disease; Research; Rett Syndrome; Role; Serotonin; Services; Sphingolipidoses; Synapses; System; Techniques; Technology; Time; Tissues; Update; computerized; gamma-Aminobutyric Acid; immunocytochemistry; lipid disorder; mass spectrometer; medical schools; morphometry; neonatal hypoxic-ischemic brain injury; neurochemistry; neuroimaging; organic acid

The Neuroscience Core was a part of our MRDDRC during the initial period of support from 1987-1992, and consisted of a synaptic neurochemistry unit directed by Dr. Joseph Coyle at Johns Hopkins Hospital and a lipid biochemistry unit directed by Dr. Yasuo Kishimoto at the Kennedy Institute. The synaptic neurochemistry core provided biochemical assays for neurotransmitter enzymes, high performance liquid chromatography (HPLC) assays for a variety of neurotransmitters including amino acids and receptor binding assays for neurotransmitter receptors in the brain. The lipid biochemistry unit provided HPLC, gas liquid chromatography (GLC) and GLC-mass spectrometer assays used to characterize inborn errors of metabolism including sphingolipidoses, peroxisomal disorders such as adrenoleukodystrophy and Refsum's disease and lysosomal disorders. Dr. Michael Johnston replaced Dr. Coyle as director of the Neuroscience Core and head of the synaptic neurochemistry unit during the next period of support from 1993-1998 after Dr. Coyle left Hopkins to become the chairman of psychiatry at Harvard Medical School. Dr. Johnston recruited Dr. Mary Blue as a coinvestigator of the sysnaptic neurochemistry core to develop a new neuroimaging unit for brain histology. During this period the synaptic neurochemistry unit continued to provide HPLC assays for neurotransmitters and expanded sophisticated imaging techniques for immunocytochemistry and analysis of neurotransmitter receptors and messenger RNA's in tissue sections using quantitative morphometric techniques. At the same time Dr. Paul Watkins replaced Dr. Kishimoto as director of the lipid neurochemistry core. This unit continued to develop advanced techniques for studying peroxisomal and related lipid disorders and added gas chromatography/mass spectrometry services for studying fatty and organic acid disorders under the direction of Dr. Richard Kelley. Leadership of the Neuroscience Core has remained stable for two additional periods of support from 1998-2008. The Core has continues to provide the most modern technology for neuroscience research including HPLC equipment, several systems for computerized morphometry and stereology, and most recently has added a confocal microscope system. Equipment in the lipid biochemistry core has also been updated to provide expanded research on peroxisomal and organic acid disorders.

在1987-1992年的最初支持期间，神经科学核心是我们MRDDRC的一部分，并且 由约翰·霍普金斯医院约瑟夫·科伊尔博士指导的突触神经化学单元和一种脂质组成 肯尼迪研究所由岸本康夫博士指导的生物化学组。突触神经化学核心 提供神经递质酶的生化分析、高效液相色谱(HPLC) 多种神经递质的测定，包括氨基酸和受体结合分析 大脑中的神经递质受体。脂类生物化学组提供高效液相、气-液色谱 (GLC)和GLC-质谱仪分析用于表征新陈代谢的先天错误，包括 鞘磷脂沉积症、肾上腺脑白质营养不良、Refsum病和溶酶体等过氧化酶体疾病 精神错乱。迈克尔·约翰斯顿博士取代科伊尔博士成为神经科学核心中心主任和 科伊尔博士离开霍普金斯大学后，在1993-1998年的下一次支持期间的突触神经化学单位 成为哈佛医学院精神病学的主席。约翰斯顿博士招募玛丽·布鲁博士为合作调查员 以开发一种新的脑组织学神经成像装置。 在此期间，突触神经化学组继续提供神经递质的高效液相分析。 以及用于免疫细胞化学和神经递质分析的扩展的尖端成像技术 使用定量形态计量学技术在组织切片中检测受体和信使RNA。同时 是时候保罗·沃特金斯博士取代岸本博士担任脂质神经化学核心主任了。本单位继续 开发研究过氧化物酶和相关的血脂紊乱及补充气体的先进技术 在指导下研究脂肪和有机酸紊乱的色谱/质谱学服务 理查德·凯利博士的。神经科学核心的领导层在另外两个时期保持稳定 1998-2008年间的支持。Core继续为神经科学提供最现代的技术 研究包括高效液相色谱设备，几个计算机形态测量和体视学系统，以及 最近又增加了一个共焦显微镜系统。脂质生化核心中的设备也已 已更新，以提供关于过氧化物酶体和有机酸紊乱的扩展研究。