Super-resolution structured illumination microscope

超分辨率结构照明显微镜

• 批准号: 8446600
• 负责人: Liza A Pon
• 金额: $ 57.92万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8446600
• 关键词: Adoption; Architecture; Cell division; Cell physiology; Cells; Cellular Structures; Cytoskeleton; Disease; Funding; Genome; Heart Diseases; Image; Inflammation; Kinetochores; Knowledge; Lateral; Life; Light; Light Microscope; Lighting; Malignant Neoplasms; Membrane; Microscope; Microscopic; Molecular; Nuclear Envelope; Organelles; Performance; Procedures; Process; Resolution; Structure; System; Techniques; United States National Institutes of Health; Universities; Work; cost; experience; instrument; light microscopy; member; migration; therapeutic development; user-friendly

DESCRIPTION (provided by applicant): This proposal is to obtain a new commercial Zeiss ELYRA S.1 Super-Resolution Structured Illumination Microscope (SR-SIM) that has unique multi-channel super- resolution capabilities for a group of cell biologists studying the molecular architecture of processes including cell division, migration, polarity and organelle dynamics and inheritance. One of the major limitations of light microscopy for the study of cell structure and function has been the inability to resolve structures below the diffraction limit imposed on optica systems. The ELYRA S.1 SR-SIM uses structured illumination to generate images that exceed the normal resolution barrier by two-fold in both lateral (X, Y) and axial (Z) directions. The ELYRA S.1 SR-SIM provides a rare combination that is not found in other commercially available SR-SIMs: superior performance (user- friendly high-resolution 3D SIM imaging in three channels, and rapid live-cell SIM imaging) and low cost. The major users of the requested instrument are all experienced cell biologists who have made extensive use of light microscopic approaches in their past work. This group seeks to use the ELYRA S.1 SR-SIM to extend their NIH-funded studies to obtain higher-resolution information on processes such as the interaction of cytoskeleton with membranes, organelle genome structure and localization, kinetochore dynamics, and nuclear envelope structure. Collaborative interactions already exist within members of this group and this is expected to facilitate adoption of procedures and techniques that will enhance the utility of the super-resolution microscope. The instrument will be administered as part of an established and well-supported imaging center at Columbia University, which will facilitate broad exposure of the instrument to additional users. The higher-resolution information obtained with the ELYRA S.1 SR- SIM should extend our knowledge of fundamental cellular processes occurring below the resolution of our existing light microscopes. This information will be helpful in identifying new avenues for the development of therapeutic approaches for the treatment of diseases such as cancer, inflammation and heart disease that result in part from deficits in the proper organization of cellular architecture.

描述（由申请人提供）：本申请是为了获得一种新的商用蔡司ELYRA S.1超分辨率结构照明显微镜（SR-SIM），该显微镜具有独特的多通道超分辨率能力，用于细胞生物学家研究细胞分裂、迁移、极性和细胞器动力学和遗传等过程的分子结构。光学显微镜研究细胞结构和功能的主要限制之一是无法分辨光学系统衍射极限以下的结构。ELYRA S.1 SR-SIM使用结构化照明来生成在横向（X， Y）和轴向(Z)方向上超过正常分辨率屏障两倍的图像。ELYRA S.1 SR-SIM提供了其他商用SR-SIM中没有的罕见组合：卓越的性能（用户友好的三通道高分辨率3D SIM成像和快速活细胞SIM成像）和低成本。所要求的仪器的主要用户都是经验丰富的细胞生物学家，他们在过去的工作中广泛使用了光显微镜方法。该小组寻求使用ELYRA S.1 SR-SIM扩展他们的nih资助的研究，以获得更高分辨率的过程信息，如细胞骨架与膜的相互作用，细胞器基因组结构和定位，着丝点动力学和核膜结构。协作互动已经存在于这个小组的成员中，这有望促进程序和技术的采用，从而提高超分辨率显微镜的实用性。该仪器将作为哥伦比亚大学一个已建立并得到良好支持的成像中心的一部分进行管理，这将有助于将仪器广泛地暴露给更多的用户。使用ELYRA S.1 SR- SIM获得的高分辨率信息应该扩展我们对现有光学显微镜分辨率以下发生的基本细胞过程的知识。这一信息将有助于确定治疗癌症、炎症和心脏病等疾病的新途径，这些疾病部分是由于细胞结构的正常组织缺陷造成的。