Shared Instrumentation Grant for Purchase of a Nikon N-SIM/N-STORM Super-Resoluti

描述(由申请人提供)：本建议书申请资金用于购买尼康N-SIM/N-STORM超分辨率显微镜系统。该仪器将SIM和Storm系统整合到一个平台中。N-SIM(结构照明显微镜)在每个维度上的分辨率都比传统的共焦显微镜高两倍。它配备了5条激光线路(458/488/515/532/561 nm)，能够进行最大厚度为20？m的3D成像。N-STORM(随机光学重建显微镜)是一种基于单分子定位的显微镜，它产生的分辨率大大提高了十倍(约。20 nm)都比共焦显微镜高 斧头。N-Storm配备了4激光照明单元(405/488/561/647 nm)和3D Storm功能。目前，石溪大学还没有超分辨率显微镜。该系统将有助于蛋白质定位和细胞结构的空间分辨率超过~200 nm的衍射限制，使研究人员能够高精度地探索与人类疾病相关的基本生物学问题。该系统的管理和操作将由一名技术专家提供，他还将协助用户制定特殊项目的染色和成像方案，并帮助调查人员分析超分辨率数据。)

DESCRIPTION (provided by applicant): This proposal requests funds for the purchase of a Nikon N-SIM/N-STORM Super-Resolution Microscope System. The instrument combines both SIM and STORM systems into one platform. The N-SIM (Structured Illumination Microscopy) achieves a resolution two times higher than that of conventional confocal microscopes in each dimension. It is equipped with 5 laser lines (458/488/515/532/561 nm) and capable of 3D imaging up to 20 ¿m thickness. The N-STORM (Stochastic Optical Reconstruction Microscopy) is a single-molecule localization-based microscope that generates a dramatically enhanced resolution that is ten times (approx. 20 nm laterally) higher than confocal microscopy in all three axes. The N-STORM comes equipped with a 4-laser illumination unit (405/488/561/647 nm) and 3D STORM capability. Currently, there is no super-resolution microscope available at Stony Brook University. The system will facilitate the spatial resolution of protein localization and cellular structures beyond the diffraction limit of ~200nm, enabling investigators to explore fundamental biological problems related to human disease at high precision. Management and operation of the system will be provided by a technical specialist, who will also assists users in developing staining and imaging protocols for special projects, as well as helping investigators in the analysis of super-resolution data. )