Union Biometrica BIOSORTER PRO large-particle flow cytometer

Union Biometrica BIOSORTER PRO 大颗粒流式细胞仪

• 批准号: 8448051
• 负责人: CHARLES F BAER
• 金额: $ 60万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-20 至 2014-06-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8448051
• 关键词: Ambystoma; Animal Model; Aplysia; Biological; Biological Models; Biology; Biomedical Research; Biotechnology; Caenorhabditis elegans; Cells; Core Facility; Drosophila genus; Embryo; Florida; Flow Cytometry; Fostering; Funding; Generations; Green Fluorescent Proteins; Human; Individual; Institution; Islets of Langerhans; Laboratories; Larva; Location; Marine Invertebrates; Measures; Microscopic; Minor; Neurons; Organism; Property; Research; Research Personnel; Resources; Sampling; Sorting - Cell Movement; System; Time; United States National Institutes of Health; Universities; instrument; particle; programs; research study; trait

DESCRIPTION (provided by applicant): A user group of five NIH-funded investigators requests funds to purchase a Union Biometrica BIOSORTER- PRO large-particle flow cytometer. A primary application of the instrument is sorting, measuring, and otherwise handling worms, specifically the common laboratory model organism Caenorhabditis elegans. The ability to handle samples in an automated, high-throughput fashion, with the additional ability to discriminate between individuals carrying fluorescent markers (e.g., green fluorescent protein, GFP), will transform both the kind and the magnitude of projects feasible to the users. The major users primarily or exclusively employ C. elegans in their research programs. The inherent power of C. elegans as a model system in biomedical research resides in its biological simplicity, its small size, and its rapid generation time. These features mean that the properties of the organism itself rarely limit the throughput of an experiment. Rather, the limitations are practical ones imposed by the inherent difficulty of scoring traits on individuals that are (almost microscopic in size. The great value of the worm sorter is to greatly increase the potential throughput of a wide variety of experiments, thereby allowing the users to leverage the inherent utility of the C. elegans system to its fullest potential. However, the instrument as outfitted her is capable of handling a diverse variety of organisms and cells, and its applications extend far beyond worm biology. An additional group of 11 minor users (most NIH- funded) have disparate applications including Drosophila, human pancreatic islets, Aplysia neurons, Axolotl embryos and marine invertebrate larvae. The instrument will be located in and managed by the University of Florida Interdisciplinary Center for Biotechnology Research Flow Cytometry core facility. There is currently no such instrument located in the state of Florida. The major users are located at the University of Florida and the University of Miami; minor users are situated both at UF and at other institutions around the state of Florida. In addition to greatly advancing the individual research programs of the users, the availability of the instrument in a central location in the state of Florida will provide an important regional resource and foster collaborative research throughout the state.

描述（由申请人提供）：一个由5名美国国立卫生研究院资助的研究人员组成的用户组申请资金购买Union Biometrica BIOSORTER- PRO大颗粒流式细胞仪。该仪器的主要应用是分类、测量和处理蠕虫，特别是常见的实验室模式生物秀丽隐杆线虫。以自动化、高通量方式处理样品的能力，以及区分携带荧光标记（例如，绿色荧光蛋白，GFP）的个体的额外能力，将改变用户可行项目的种类和规模。主要用户主要或专门使用秀丽隐杆线虫在他们的研究计划。秀丽隐杆线虫作为生物医学研究模型系统的内在力量在于其生物简单性、体积小、生成时间短。这些特征意味着生物体本身的特性很少限制实验的吞吐量。更确切地说，这些限制是实际的，因为对几乎是微观大小的个体进行特征评分的固有困难。蠕虫分类器的巨大价值是大大增加了各种实验的潜在吞吐量，从而允许用户利用秀丽隐杆线虫系统的固有效用，以充分发挥其潜力。然而，她配备的仪器能够处理各种各样的生物体和细胞，其应用远远超出了蠕虫生物学。另外一组11个小用户（大多数是NIH资助的）有不同的应用，包括果蝇、人类胰岛、美洲鳄神经元、蝾螈胚胎和海洋无脊椎动物幼虫。该仪器将位于佛罗里达大学跨学科生物技术研究中心的流式细胞仪核心设施内并由其管理。目前在佛罗里达州还没有这样的仪器。主要用户位于佛罗里达大学和迈阿密大学；未成年用户位于佛罗里达大学和佛罗里达州周围的其他机构。除了大大推进个人的研究计划的用户，仪器的可用性在一个中心位置