WormGuides: a resource for Global Understanding in Dynamic Embryonic Systems

描述（由申请人提供）：秀丽隐杆线虫是了解广泛的人类疾病的关键动物模型。关于秀丽隐杆线虫的系统级知识的生成和开放共享极大地促进了线虫的生产力。在这里，我们提出WormGUIDES（全球理解动态胚胎系统），一个新的资源，将导致创建和共享任何动物胚胎发生和神经发育的第一个地图集。虫指南将产生一个互动的4D核位置图谱，从受精卵到孵化。我们设想这将成为整个社区使用的日常参考工具，用于指导细胞鉴定，量化发育过程和可视化胚胎的新生模式和对称性。虫指南也将导致一个技术管道为创建一个神经发育地图集。秀丽隐杆线虫是唯一具有已知连接体的动物，通过WormGUIDES创建的工具将能够检查这种连接体在发育过程中如何出现。WormGUIDES将把这些关于秀丽隐杆线虫胚胎发生的新知识整合到现有的和广泛使用的资源中，如WormAtlas和WormBase。蠕虫指南的影响将是变革性的，它提供了一个全面的资源，将使我们能够检查目前难以接近的神经发育方面，同时进一步加深我们对胚胎发生的理解，并提高秀丽隐杆线虫作为模式生物的价值。

DESCRIPTION (provided by applicant): C. elegans is a key animal model for understanding a broad spectrum of human diseases. Productivity around C. elegans has been greatly facilitated by the generation and open sharing of systems-level knowledge of the organism. Here we propose WormGUIDES (Global Understanding in Dynamic Embryonic Systems), a novel resource that will result in the creation and sharing of the first atlas of embryogenesis and neurodevelopment for any animal. WormGUIDES will result in an interactive 4D atlas of nuclear positions, from zygote until hatching. We envision this will become an everyday reference tool used by the entire community to guide cell identification, quantify developmental processes and visualize nascent patterns and symmetries in the embryo. WormGUIDES will also result in a technical pipeline for the creation of a neurodevelopmental atlas. C. elegans is the only animal with a known connectome, and the tools created through WormGUIDES will enable examination of how this connectome emerges during development. WormGUIDES will integrate this newly generated knowledge of C. elegans embryogenesis to existing and widely used resources, such as WormAtlas and WormBase. The impact of WormGUIDES will be transfomative, providing a comprehensive resource that will enable examination of currently inaccessible aspects of neurodevelopment, while furthering our understanding of embryogenesis and enhancing the value of C. elegans as a model organism.