IMAGING CORE

核心A(成像)对于宫内胎儿和畸胎瘤的MRI成像是至关重要的，并最终用于跟踪 移植的干细胞。体外实时和激光扫描共聚焦、定量FRAP、广场和电子 还向所有三个分项目提供了显微镜，以评估多能性活细胞指标的动态变化。 HESC、nhpESC和PG细胞，以便更好地确定多能性状态的特征。成像核心提供 对体外、子宫和体内干细胞动力学的异常高灵敏度检测，对于每一种 研究项目。为了确保成像核心的最大效用，我们将结合体内非侵入性的优点 用常规、共聚焦和电子显微镜进行体外成像的磁共振成像。在宏观整体上 在动物层面，我们将利用新的小动物成像技术，如核磁共振。影像的主要作用 核心将是：对怀孕的猴子进行纵向核磁共振研究，并监测存活率和表型 嵌合体胎儿和胎盘在整个妊娠过程中的差异应用高分辨率3D MRI对固定和活体进行成像 胚胎来源的NHP胚胎，以检查干细胞的贡献；并监测体内畸胎瘤的形成。 目的1.成像核心将为所有三个项目提供全身无创功能成像。核心是 负责跟踪ES和EG嵌合胚胎中的细胞谱系贡献以及移植的HES的命运 细胞。该核心还将使用高分辨率核磁共振成像来绘制胎儿发育和畸胎瘤形成的地图，并执行 切除畸胎瘤前的磁共振显微镜检查。这一核心还将致力于开发 以及优化体内干细胞的跟踪技术，包括用于磁共振的ES和EG细胞的SPIO标记。 目标2.成像核心将为固定的、免疫细胞化学、活的 细胞共聚焦、实时动态和电子显微镜分析细胞结构和动力学 分化的人类胚胎干细胞，特别是活的和固定的多潜能标记分析，以更好地表征 未分化的人和非人灵长类ES和EG细胞。该核心还将执行对ES的基础研究 和EG细胞对嵌合胚胎的贡献以及ES和EG细胞的印记状态，以及 畸胎瘤和类胚体的免疫细胞化学和免疫组织化学。

Core A (Imaging) is vital for the MRI imaging of fetuses in utero and teratomas, and ultimately for tracking transplanted stem cells. In vitro real-time and laser-scanning confocal, quantitative FRAP, wide-field and electron microscopy are also provided to all three subprojects to evaluate the dynamics of live cell indicators of pluripotency in hESC, nhpESC and PG cells in order to better define characteristics of the pluripotent state. The imaging core provides exceptional high-sensitivity detection of stem cell dynamics in vitro, in utero and in vivo, essential for each of the research projects. To ensure maximum utility of the Imaging Core, we will combine the strengths of in vivo noninvasive MRI with in vitro imaging by conventional, confocal, and electron microscopy. At the macroscopic whole animal level, we will utilize new small animal imaging technologies, such as MRI. The primary roles of the Imaging Core will be to: perform longitudinal MRI studies of pregnant monkeys and monitor viability and phenotypic differences of the chimeric fetus and placenta throughout pregnancy; employ high-resolution 3D MRI of fixed and live ES-derived NHP embryos to examine stem cell contributions; and monitor teratoma formation in vivo. Aim 1. The imaging core will provide total body non-invasive functional imaging to all three projects. The core is responsible for tracking cell lineage contributions in ES and EG chimeric embryos and the fates of transplanted hES cells. The core will also use high resolution MRI to map fetal development and teratoma formation, and perform magnetic resonance microscopy on excised teratomas prior to sectioning. This core will also work towards developing and optimizing techniques for the tracking of stem cells in vivo including SPIO labeling of ES and EG cells for MRI. Aim 2. The imaging core will provide instrumentation, oversight and guidance for fixed, immunocytochemistry, live cell confocal, real-time dynamic and electron microscopy for assaying cell structure and dynamics in pluripotent and differentiated human embryonic stem cells, especially live and fixed pluripotent marker assays to better characterize undifferentiated human and nonhuman primate ES and EG cells. This core will also perform fundamental studies of ES and EG cell contributions to chimeric embryos and imprinting status of ES and EG cells, as well as immunocytochemistry and immunohistochemistry of teratomas and embryoid bodies.