Multi-functional particles for stem cell isolation and expansion

用于干细胞分离和扩增的多功能颗粒

• 批准号: 8644605
• 负责人: Wenjing Hu
• 金额: $ 20.03万
• 依托单位: PROGENITEC, INC.
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-11 至 2017-07-10
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8644605
• 关键词: 3-Dimensional; Allylamine; Antibodies; Applied Research; Architecture; Atherosclerosis; Basic Science; Binding; Blood; Blood Vessel Prosthesis; CD34 gene; Cardiovascular Diseases; Cell Culture Techniques; Cell Separation; Cell Therapy; Cell Transplantation; Cell-Matrix Junction; Cells; Centrifugation; Chemicals; Development; Disease; Effectiveness; Enzymes; Evaluation; Goals; Human; In Vitro; Knowledge; Magnetism; Mechanics; Medical; Medical Research; Methods; Modeling; Myocardial Infarction; Outcome; Phase; Polymers; Polystyrenes; Preclinical Drug Evaluation; Process; Property; Research; Small Business Technology Transfer Research; Source; Stem Cell Research; Stem cells; Structure; Surface; System; Temperature; Therapeutic; Tissues; Work; bone; cell type; cellular engineering; design; improved; innovation; magnetic cell separation; nanoparticle; novel; particle; peripheral blood; phase 1 study; poly-N-isopropylacrylamide; public health relevance; tool

DESCRIPTION (provided by applicant): The objective of this STTR Phase I proposal is to develop novel multi-layer microparticles and demonstrate their feasibility to isolate and expand stem cells for medical research and therapeutic applications. In recent years, stem cells have been used in a wide variety of applications, including cell- based therapies, drug screening, and models to understand the disease development. Unfortunately, many of these applications require large number of stem cells which cannot be produced by most of the current cell isolation and culture methods. The limitations of the current methods include the use of harmful chemicals and enzymes for cell isolation and expansion, and harsh shear forces from centrifugation during cell passaging process. To overcome these drawbacks, we will develop novel multi-layer microparticles that allow stem cell isolation via magnetic separation, cell attachment using a temperature-sensitive mechanism that binds cells at 370C, cell expansion via 3-D structural architecture, and cell detachment by a temperature-sensitive mechanism to release cells bound to the surface at room temperature. These microparticles will provide "all-in-one pot" for cell isolation, expansion, and detachment of stem cells for use in medical applications without the use of harmful chemicals and mechanical forces. For the phase I study, we will formulate and characterize these multilayer microparticles (Aim 1). The microparticles will then be evaluated for their effectiveness in isolation, expansion, and detachment of stem cells, including endothelial progenitor cells (EPCs) from human blood (Aim 2). The successful completion of this proposal may provide a means to effectively isolate and expand various cell types in addition to stem cells. The knowledge gained from this research should significantly improve our understanding of magnetic cell separation, stem cell expansion, and microparticles for cellular engineering applications.

描述（由申请人提供）：本STTR I期提案的目的是开发新型多层微粒，并证明其分离和扩增干细胞用于医学研究和治疗应用的可行性。近年来，干细胞已被用于各种各样的应用，包括基于细胞的治疗、药物筛选和用于了解疾病发展的模型。不幸的是，这些应用中的许多需要大量的干细胞，这不能通过大多数当前的细胞分离和培养方法产生。目前方法的局限性包括使用有害的化学物质和酶进行细胞分离和扩增，以及在细胞传代过程中离心产生的苛刻剪切力。为了克服这些缺点，我们将开发新型的多层微粒，其允许通过磁性分离进行干细胞分离，使用在370 ℃下结合细胞的温度敏感机制进行细胞附着，通过3-D结构架构进行细胞扩增，以及通过温度敏感机制进行细胞分离以在室温下释放结合到表面的细胞。这些微粒将为干细胞的细胞分离、扩增和分离提供“一体化锅”，用于医疗应用，而不使用有害的化学物质和机械力。对于第一阶段的研究，我们将制定和表征这些多层微粒（目标1）。然后将评价微粒在分离、扩增和分离干细胞（包括来自人血液的内皮祖细胞（EPC））中的有效性（目的2）。该提案的成功完成可能提供一种有效分离和扩增除干细胞外的各种细胞类型的手段。从这项研究中获得的知识应该会显着提高我们对磁性细胞分离、干细胞扩增和细胞工程应用微粒的理解。