ShEEP Request for High-throughput Single Cell Genomics Instrumentation

ShEEP 请求高通量单细胞基因组学仪器

基本信息

  • 批准号:
    9795153
  • 负责人:
  • 金额:
    --
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2019
  • 资助国家:
    美国
  • 起止时间:
    2019-01-01 至 2019-09-30
  • 项目状态:
    已结题

项目摘要

In this Veterans Affairs ORD Shared Equipment Evaluation Program (ShEEP) application, we are requesting instrumentation for high throughput single-cell genomics (Fluidigm C1 Single-Cell Auto Prep System, BioMark, and Illumina NextSeq 550 Sequencing System) to evaluate cell-specific genomic, epigenetic, and transcriptomic variations in diseases of high relevance to the VA mission. This instrumentation will dramatically enhance Veterans Affairs (VA) and non-VA investigators to investigate mechanisms of disease progression at the level of single cells. Although dramatic advances have been made in the last decade in our ability to process and sequence DNA and RNA in bulk populations of cells, until recently our understanding of significant variations in genomic and transcriptomic information at the level of the single cell has been limited. For instance, major insights into fundamental biological processes such as normal development and tumor evolution secondary to oncogenic potential of single cells can only be garnered from a detailed understanding of genomic, epigenomic, and transcriptional variation at the single-cell level. In addition, certain pathogenic cells are so rare that single-cell approaches become essential to their identification and characterization. Our Genomics Core is part of an ongoing collaboration to enhance the academic and administrative ties between the Northport Veterans Affairs Medical Center (NVAMC) and its affiliated Stony Brook University School of Medicine (SBUSM). This new instrumentation will be housed in NVAMC and this strategic location allows easy access to the instruments for both VA and SBUSM investigators. The location of this Core will encourage further collaboration between VA investigators at Northport and their University colleagues at Stony Brook. Technical support is available through the Genomics Core on a fee-for-service basis to support both VA researchers and their collaborators. In addition, we have assembled a team of experts for bioinfomatics support. Many of the VA investigators including Drs. Mallipattu, Lin, McKinnon, Rohatgi, Miller, Arvanian, Del Poeta, and Obeid, whose research are supported by Merit Review Awards, and would significantly benefit from the acquisition and utilization of this instrumentation for high throughput single-cell genomics. Trained personnel in the Core can operate the instrument and provide technical support and we have outlined a plan to purchase service contracts to maintain the instrument. In this ShEEP application for high-throughput single cell genomics instrumentation, we also describe in detail the specifics of the instrumentation, justification of need, demonstrate the technical expertise to support the usage of the equipment, descriptions of the major and minor users, and the organizational plan for the utilization of the instrumentation. The long-term impact of this single-cell analysis instrumentation is that it will enhance the impact and further our currently funded VA Merit Review Awards. In addition, results acquired from single-cell genomics will generate new hypothesis and provide new avenues for future investigations in diseases that are highly relevant to the VA mission.
在退伍军人事务部共享装备评估项目(ShEEP)申请中,我们是

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Sandeep K Mallipattu其他文献

The Prevalence of Post-Acute Sequelae of COVID-19 in Solid Organ Transplant Recipients: Evaluation of Risk in the National COVID Cohort Collaborative (N3C).
实体器官移植受者中 COVID-19 急性后遗症的患病率:国家 COVID 队列协作组织 (N3C) 的风险评估。
  • DOI:
    10.1016/j.ajt.2024.06.001
  • 发表时间:
    2024
  • 期刊:
  • 影响因子:
    0
  • 作者:
    A. Vinson;Makayla Schissel;A. Anzalone;Ran Dai;E. French;A. Olex;Stephen B. Lee;Michael Ison;R. Mannon;A. Wilcox;Adam M. Lee;Alexis Graves;A. Anzalone;A. Manna;Amit Saha;A. Olex;Andrea Zhou;Andrew E. Williams;Andrew Southerland;A. Girvin;Anita Walden;Anjali A. Sharathkumar;B. Amor;Benjamin Bates;Brian Hendricks;Brijesh Patel;Caleb Alexander;Carolyn T Bramante;C. Ward‐Caviness;C. Madlock;Christine Suver;C. Chute;Christopher Dillon;Chunlei Wu;Clare Schmitt;Cliff Takemoto;D. Housman;D. Gabriel;David A. Eichmann;Diego Mazzotti;Don Brown;Eilis Boudreau;Elaine Hill;Elizabeth Zampino;E. Marti;Emily Pfaff;E. French;F. Koraishy;Federico Mariona;Fred Prior;G. Sokos;Greg Martin;H. Lehmann;Heidi Spratt;Hemalkumar Mehta;Hongfang Liu;Hythem Sidky;J. Hayanga;Jami D. Pincavitch;Jaylyn F. Clark;Jeremy Harper;Jessica Islam;Jin Ge;J. Gagnier;J. Saltz;J. Saltz;Johanna J. Loomba;Jon D. Buse;Jomol P Mathew;J. Rutter;J. McMurry;Justin Guinney;J. Starren;Kay Crowley;K. Bradwell;Kellie M. Walters;K. Wilkins;Kenneth R. Gersing;K. Cato;Kimberly Murray;K. Kostka;Lavance Northington;Lee A. Pyles;Leonie Misquitta;Lesley Cottrell;L. Portilla;Mariam Deacy;Mark Bissell;M. Clark;M. Emmett;M. Saltz;M. Palchuk;Melissa A. Haendel;Meredith Adams;Meredith Temple;Michael G. Kurilla;Michele Morris;N. Qureshi;Nasia Safdar;Nicole Garbarini;Noha Sharafeldin;O. Sadan;P. A. Francis;P. W. Burgoon;Peter Robinson;Philip R. O. Payne;Rafael Fuentes;R. Jawa;Rebecca Erwin;Rena C Patel;Richard A. Moffitt;R. Zhu;R. Kamaleswaran;R. Hurley;Robert T. Miller;S. Pyarajan;Sam G. Michael;Samuel Bozzette;Sandeep K Mallipattu;Satyanarayana Vedula;Scott A. Chapman;Shawn T O'Neil;Soko Setoguchi;Stephanie S. Hong;Steve Johnson;Tellen D. Bennett;Tiffany J. Callahan;Umit Topaloglu;Usman Sheikh;Valery Gordon;V. Subbian;Warren Kibbe;Wenndy Hernandez;Willarene P. Beasley;W. Cooper;W. Hillegass;X. Zhang
  • 通讯作者:
    X. Zhang

Sandeep K Mallipattu的其他文献

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{{ truncateString('Sandeep K Mallipattu', 18)}}的其他基金

Single-cell Cyclic Multiplex in Situ Tagging to Advance Kidney Research
单细胞循环多重原位标记促进肾脏研究
  • 批准号:
    10790122
  • 财政年份:
    2023
  • 资助金额:
    --
  • 项目类别:
Small Molecule KLF15 Agonists for Kidney Disease
治疗肾脏疾病的小分子 KLF15 激动剂
  • 批准号:
    10553107
  • 财政年份:
    2021
  • 资助金额:
    --
  • 项目类别:
Small Molecule KLF15 Agonists for Kidney Disease
治疗肾脏疾病的小分子 KLF15 激动剂
  • 批准号:
    10117332
  • 财政年份:
    2021
  • 资助金额:
    --
  • 项目类别:
Small Molecule KLF15 Agonists for Kidney Disease
治疗肾脏疾病的小分子 KLF15 激动剂
  • 批准号:
    10359057
  • 财政年份:
    2021
  • 资助金额:
    --
  • 项目类别:
Role of KLF15 in proximal tubule metabolism
KLF15 在近曲小管代谢中的作用
  • 批准号:
    10481366
  • 财政年份:
    2018
  • 资助金额:
    --
  • 项目类别:
Transcriptional control of mitochondrial function by KLF6 in diabetic kidney disease
KLF6 在糖尿病肾病中对线粒体功能的转录控制
  • 批准号:
    10400042
  • 财政年份:
    2017
  • 资助金额:
    --
  • 项目类别:
Transcriptional control of mitochondrial function by KLF6 in diabetic kidney disease
KLF6 在糖尿病肾病中对线粒体功能的转录控制
  • 批准号:
    9918361
  • 财政年份:
    2017
  • 资助金额:
    --
  • 项目类别:
Transcriptional control of mitochondrial function by KLF6 in diabetic kidney disease
KLF6 在糖尿病肾病中对线粒体功能的转录控制
  • 批准号:
    9286505
  • 财政年份:
    2017
  • 资助金额:
    --
  • 项目类别:
The Role of KLF15 as a transcriptional regulator of podocyte differentiation
KLF15 作为足细胞分化转录调节因子的作用
  • 批准号:
    8750137
  • 财政年份:
    2014
  • 资助金额:
    --
  • 项目类别:
The Role of KLF15 as a transcriptional regulator of podocyte differentiation
KLF15 作为足细胞分化转录调节因子的作用
  • 批准号:
    8916713
  • 财政年份:
    2014
  • 资助金额:
    --
  • 项目类别:

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