ShEEP Request for Chromium System

ShEEP 对 Chromium 系统的请求

• 批准号: 9796800
• 负责人: Salman Azhar
• 金额: --
• 依托单位: VETERANS ADMIN PALO ALTO HEALTH CARE SYS
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9796800
• 关键词: Affect; Aging; Autoimmune Diseases; Biological; Biological Process; Biology; Blood Vessels; Bone Diseases; Cancer Model; Cells; Chromium; Computer software; DNA; DNA analysis; Data; Degenerative polyarthritis; Development; Disease; Disease model; Encapsulated; Event; Gel; Gene Expression; Generations; Genes; Genome; Genomic DNA; Genomics; Gulf War; Haplotypes; Health; Healthcare; Heterogeneity; Human; Human Genome; Immune; Immune response; Individual; Injury; Knowledge; Label; Libraries; Link; Metabolic Diseases; Metabolic syndrome; Mission; Molecular; Molecular Weight; Muscle; Nerve Degeneration; Obesity; Oils; Pain; Pathogenesis; Pathologic; Patient Care; Phase; Population; Preparation; Process; Radiation; Reagent; Research; Research Personnel; Rodent; Rotavirus Infections; Sampling; Sheep; Stem cells; Steroid biosynthesis; Structure; System; T cell regulation; T-Cell Activation; Technology; Variant; Veterans; Whole-Genome Shotgun Sequencing; Work; adipokines; artery stenosis; bone healing; cell type; design; exome; genome analysis; health administration; improved; innovation; insertion/deletion mutation; instrument; lipid metabolism; lipid transport; nanolitre; non-alcoholic fatty liver disease; research study; targeted treatment; wound

The 10x Genomics Chromium System is an important instrument for our research. The 10X Genomics Chromium System is comprised of the Chromium Controller, Chromium i7 Multiplex kit, Chromium Single Cell 3' Library and Gel Bead Kit, and the Chromium Single Cell A Chip kit. These components are used together to perform Genome “linked read” library preps and sequencing. These components will only work with the Chromium Controller and cannot be used independently. There are many investigators that are researching specific single cell types to elucidate mechanisms of action of various biological processes. This is hard to do in primary cells as there are many cell types that must be isolated from one another. This system can identify single cells, so different cell populations can be studied without other cell types confounding the results. This Single Cell and DNA Analysis 10X Genomics™ consists of 10x GemCode™ that fuels Chromium System with an innovative reagent delivery of previously inaccessible genomic information at a massive rate and scale. The Chromium Controller can quickly encapsulate individual cells or genomic DNA into 100,000s to 1,000,000s of uniquely addressable partitions in minutes, each containing an identifying barcode for downstream genomic analysis with the Chromium Software Suite. It will allow us to explore how aging and other diseases conditions impact major classes of structural variants (SVs) like deletions, inversions and translocations, and also allows the sequencing of genes inaccessible to short-read sequencers. 10X Genomics™ will also allow analysis of Phase SNVs, indels and SVs across > 10 Mb haplotype blocks. Finally, it will allow generation of high-quality libraries from as little as 1 ng of genomic DNA. This technology is extremely important to VA research. There are fourteen principle investigators at VAPAHCS who will apply this technology in their research to analyze gene expression at a single cell level to elucidate mechanisms of aging, steroidogenesis, wound/bone healing, lipid metabolism, cellular immune responses, T cell activation, bone diseases, obesity, Gulf War Illness, as well as many other metabolic diseases and conditions that particularly affect the Veterans population. The knowledge gained from these projects will enhance our understanding of the molecular events connected with the pathological conditions and pathogenesis of various conditions that affect veterans'' health.

10倍基因组铬系统是我们研究的重要工具。10倍 基因组学铬系统由铬控制器Chromium i7 Multiplex组成 试剂盒、铬单细胞3‘库和凝胶珠试剂盒，以及铬单细胞A芯片试剂盒。 这些组件一起用于执行基因组“链接读”文库的准备和 测序。这些组件只能与Chromium控制器一起使用，不能使用 独立的。有许多研究人员正在研究特定的单细胞类型以 阐明各种生物过程的作用机制。在原代细胞中很难做到这一点 因为有许多必须彼此隔离的细胞类型。该系统可以识别 单个细胞，因此可以研究不同的细胞群，而不需要其他细胞类型来混淆 结果。单细胞和脱氧核糖核酸分析10X基因组™由10倍GemCode™组成， 用一种创新的试剂输送以前无法获得的基因组来为铬系统提供燃料 以海量的速度和规模传播信息。Chromium控制器可以快速封装 单个细胞或基因组DNA进入100,000到1,000,000个唯一可寻址的分区 分钟，每个包含用于下游基因组分析的识别条形码 Chrome软件套件。它将使我们能够探索衰老和其他疾病是如何 影响主要类别的结构变异(SVS)，如缺失、倒位和易位，以及 还允许对短读测序仪无法获得的基因进行测序。10倍基因组学™将 还允许跨&gt；10 Mb单倍型块分析相位SNV、INDELS和SVS。最后，它 将允许从仅1 ng的基因组DNA中生成高质量的文库。 这项技术对退伍军人管理局的研究极其重要。有14名首席调查员 将在他们的研究中应用这项技术来分析基因表达 单细胞水平阐明衰老、类固醇生成、伤口/骨愈合、脂质的机制 新陈代谢、细胞免疫反应、T细胞活化、骨病、肥胖、海湾战争 疾病，以及许多其他代谢疾病和疾病，特别影响 退伍军人人口。从这些项目中获得的知识将增进我们的理解 与各种疾病的病理状态和发病机制有关的分子事件 影响退伍军人健康的条件。