Vitamin D Deficiency Leads to Increased Intra‐Prostatic Androgens in African American Men

项目总结 这项建议是为促进与健康相关的研究的多样性而设立的研究附录，以 R21 CA231610。我建议支持实验室的三年级研究生杰森·加西亚先生， 在这个项目上工作。加西亚先生是墨西哥裔美国人，他的职业目标是“挣教员” 一所大学的职位，为寻求在美国接受教育的代表人数不足的学生服务 同时继续调查癌症的差异。他设计了一份增刊 旨在补充R21的目标。 目的S1：定量巨蛋白介导的血清球蛋白、维生素D结合 蛋白质和性激素结合球蛋白，进入前列腺细胞和组织。 这些实验使用了体外组织切片和前列腺的尖端方法 有机化合物为Jason提供与癌症相关的患者衍生临床前模型方面的专业知识 差异研究。此外，通过蛋白质操作获得的技术技能， 共聚焦显微镜和信息学量化将拓宽他在 实验室环境。 培训：完成加西亚先生的目标，为他成为有竞争力的博士后做好准备 他最后两年的培训将集中在癌症差异研究方面的卓越表现， 对本科生或高中生的指导，以及概述的几个教学要素 在提案中。

PROJECT SUMMARY This proposal is for a Research Supplements to Promote Diversity in Health-Related Research to R21 CA231610. I propose to support a third year graduate student in the lab, Mr. Jason Garcia, to work on this project. Mr. Garcia is Mexican American whose career goal is “to earn a faculty position at a university catering to underrepresented students seeking an education in the STEM fields while continuing to investigate cancer disparities.” He has designed a supplement aim to complement the Aims of the R21. Aim S1: To quantify megalin-mediated import of serum globulins, vitamin D binding protein and sex-hormone binding globulin, into prostate cells and tissues. These experiments use the cutting edge methods of ex vivo tissue slices and prostate organoids to provide Jason expertise in patient-derived preclinical models relevant to cancer disparities research. Additionally, the technical skills gained with the protein manipulation, confocal microscopy and informatics quantitation will broaden his breadth of expertise in the laboratory setting. Training: To accomplish Mr. Garcia's goal and prepare him for a competitive postdoctoral position, the last 2 years of his training will focus on excellence in cancer disparities research, mentoring of undergraduate or high school students, as well as several didactic elements outlined in the proposal.