Metabolomics Core

摘要 在过去的二十年里，代谢组学领域由于以下方面的重大进展而发生了巨大的变化 分析技术和信息学。然而，这些进步并没有被有效地用来增强我们的 了解细菌生理学或宿主与病原体的相互作用。拟议的新陈代谢组学核心将 通过协助本PPG中涉及生理的各种项目来解决这一不足 金黄色葡萄球菌及其与宿主的相互作用。具体地说，代谢组学核心将标准化 涉及提取细胞内和排泄代谢物的研究方法，建立了一家公司 根据长期存在的生化原理解释受影响的代谢途径的理论基础， 设计先进的方案来检测独特的细胞代谢物，并利用示踪剂代谢物(例如 C13标记的代谢物)来研究特定的代谢途径。这些目标将在以下情况下实现 收购最先进的Q激活型气相色谱/质谱仪 由于其高分辨率精确质量(HRAM)轨道捕获功能，可实现目标分析和非目标分析 技术GC-Platform不仅将补充现有的LC-MS(LC-MS) 基于MS/MS)的方法目前正被核心使用，但对于旨在 通过代谢途径确定碳通量测量。我们预计代谢组学的核心 将是与该PPG相关的项目的成功结果不可或缺的一部分，而且将是一个关键 为更广泛的葡萄球菌研究社区提供的资源。

ABSTRACT In the last two decades, the field of metabolomics has evolved tremendously due to significant advances in analytical technology and informatics. However, these advances have not efficiently been used to augment our understanding of bacterial physiology or host-pathogen interactions. The proposed Metabolomics Core will address this deficiency by assisting the various projects in this PPG that deal with the physiology of Staphylococcus aureus and its interaction with the host. Specifically, the Metabolomics Core will standardize research methodology involved with the extraction of intracellular and excreted metabolites, establish a firm rationale for the interpretation of affected metabolic pathways based on long-standing biochemical principles, design advanced protocols for the detection of unique cellular metabolites and utilize tracer metabolites (e.g. C13- labelled metabolites) to investigate specific metabolic pathways. These aims will be achieved following the acquisition of the state-of-the-art Q Exactive Gas Chromatography (GC-MS/MS) mass spectrometer which enables both targeted and untargeted analyses due to its High-Resolution Accurate Mass (HRAM) Orbitrap technology. The GC-platform will not only complement existing Liquid Chromatography-Mass Spectrometry (LC- MS/MS) based methods currently being utilized by the core but will also uniquely be useful for studies aimed at determining carbon flux measurements through metabolic pathways. We anticipate that the Metabolomics Core will be indispensable for the successful outcome of projects associated with this PPG and further, will be a critical resource for the broader staphylococcal research community.