Metabolomics Core

摘要 在过去的二十年里，代谢组学领域由于以下方面的重大进展而发生了巨大的发展： 分析技术和信息学。然而，这些进步并没有被有效地用于增强我们的 了解细菌生理学或宿主与病原体相互作用。代谢组学核心将 通过协助本PPG中处理以下生理学问题的各种项目来解决这一不足： 金黄色葡萄球菌及其与宿主的相互作用。具体来说，代谢组学核心将标准化 研究方法涉及细胞内和排泄代谢物的提取，建立一个公司 基于长期存在的生物化学原理解释受影响代谢途径的基本原理， 设计用于检测独特的细胞代谢物的先进方案，并利用示踪代谢物（例如， C13标记的代谢物），以研究特定的代谢途径。这些目标将在 采购最先进的Q Exactive气相色谱（GC-MS/MS）质谱仪， 由于其高分辨率精确质量（HRAM）轨道阱， 技术. GC平台不仅将补充现有的液相色谱-质谱法（LC-MS）， MS/MS）为基础的方法，目前正在使用的核心，但也将是唯一有用的研究，旨在 通过代谢途径测定碳通量。我们预计代谢组学核心 对于与本PPG相关的项目的成功结果是不可或缺的，而且， 更广泛的葡萄球菌研究社区的资源。

ABSTRACT In the last two decades, the field of metabolomics has evolved tremendously due to significant advances in analytical technology and informatics. However, these advances have not efficiently been used to augment our understanding of bacterial physiology or host-pathogen interactions. The proposed Metabolomics Core will address this deficiency by assisting the various projects in this PPG that deal with the physiology of Staphylococcus aureus and its interaction with the host. Specifically, the Metabolomics Core will standardize research methodology involved with the extraction of intracellular and excreted metabolites, establish a firm rationale for the interpretation of affected metabolic pathways based on long-standing biochemical principles, design advanced protocols for the detection of unique cellular metabolites and utilize tracer metabolites (e.g. C13- labelled metabolites) to investigate specific metabolic pathways. These aims will be achieved following the acquisition of the state-of-the-art Q Exactive Gas Chromatography (GC-MS/MS) mass spectrometer which enables both targeted and untargeted analyses due to its High-Resolution Accurate Mass (HRAM) Orbitrap technology. The GC-platform will not only complement existing Liquid Chromatography-Mass Spectrometry (LC- MS/MS) based methods currently being utilized by the core but will also uniquely be useful for studies aimed at determining carbon flux measurements through metabolic pathways. We anticipate that the Metabolomics Core will be indispensable for the successful outcome of projects associated with this PPG and further, will be a critical resource for the broader staphylococcal research community.