Intestinal Triacylglycerol Metabolism and Energy Balance

肠道三酰甘油代谢和能量平衡

• 批准号: 8638954
• 负责人: Chi- Liang Eric Yen
• 金额: $ 32.3万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8638954
• 关键词: 2-acylglycerol O-acyltransferase; Acyl Coenzyme A; Address; Affect; Apolipoproteins B; Assimilations; Blood Circulation; Body fat; Chylomicrons; Diet; Dietary Fats; Distal; Duodenum; Eating; Endocrine Glands; Energy Metabolism; Enterocytes; Enteroendocrine Cell; Enzymes; Epidemic; Essential Fatty Acids; Fatty Acids; Fatty acid glycerol esters; Gastric Inhibitory Polypeptide; Genetic Models; Glucagon; Goals; Health; Homeostasis; Hormones; Human; Infusion procedures; Insulin Resistance; Intestines; Isotopes; Knowledge; Lead; Lipids; Lipoproteins; Lymph; Measures; Mediating; Metabolic; Metabolic Diseases; Metabolism; Molecular; Mus; Nutrient; Nutritional status; Obesity; Peripheral; Phenotype; Physiological Processes; Play; Process; Research; Resistance; Role; Signal Transduction; Source; Testing; Time; Tissues; Tracer; Triglycerides; Universities; Vitamins; Weight Gain; Work; absorption; body system; combat; detection of nutrient; energy balance; feeding; improved; insight; lipid metabolism; novel; particle; public health relevance; response; therapeutic target; therapy development; uptake

DESCRIPTION (provided by applicant): The intestine plays a pivotal role in lipid metabolism and energy balance. It is the portal for dietary nutrients, the source of apolipoprotein B-containing chylomicra, and an endocrine organ that signals current nutritional status to other tissues to maintain homeostasis and promote metabolic efficiency. However, many molecular mechanisms involved in these processes remain elusive. The long-term goal of our research is to understand how intestinal lipid processing regulates systemic lipid metabolism and energy balance. The overall objective of this proposal is to elucidate the mechanism(s) by which acyl CoA:monoacylglycerol acyltransferase-2 (MGAT2) regulates whole body energy balance. Mice deficient in MGAT2 (Mgat2-/- mice) display a remarkable resistance to obesity and related metabolic disorders induced by high-fat feeding. In contrast to the well- established role of the intestine in regulating food intake and nutrient assimilation, the phenotype of Mgat2-/- mice suggests a previously unrecognized role of the intestine in modulating energy expenditure. Among known MGAT enzymes, MGAT2 is highly expressed in the intestine of human and mouse. MGAT activity is best known for its role in the absorption of dietary fat, because it catalyzes triacylglycerol re-synthesis, a required step for the formation of chylomicra, which deliver dietary fat to peripheral tissues. Despite consuming and absorbing normal amounts of fat, Mgat2-/- mice are protected from excessive weight gain on a high-fat diet. Compared to their control littermates, however, these mice expend significantly more energy. The difference in energy expenditure increases, as dietary fat increases. Although Mgat2-/- mice absorb normal quantities of fat, more of the dietary fat is absorbed from the distal intestine, thereby delaying the entry of dietary fat into the circulation. In addition, their postprandial levels of glucose-dependent insulinotropic peptide (GIP) are lower, whereas glucagon-like peptide1 (GLP1) levels are higher than in controls. Both gut hormones can affect energy balance. Thus, we hypothesize that MGAT2 coordinates the uptake and processing of lipid for chylomicron formation in enterocytes and modulates the secretion of GIP and GLP1 from enteroendocrine cells. As such, intestinal MGAT2 directs the delivery of dietary fat toward storage for efficient assimilation of this calorie-dense nutrient. To test our hypothesis, in Aim 1, we will elucidate the role of MGAT2 in intestinal lipid metabolism, chylomicron formation, and gut hormone release. In Aim 2, we will determine the functional consequences of lacking MGAT2 on the distribution of dietary fat. In Aim 3, we will assess the overall impact of intestinal MGAT2 on systemic energy balance using mice that express MGAT2 only in the intestine and mice that lack MGAT2 in the same intestine-specific manner. By completing these aims, we will advance our understanding of how intestinal lipid metabolism modulates systemic energy balance, which would be important, because it would build a new paradigm for understanding fat assimilation, a fundamental physiological process that is crucial for survival during lean times but may lead to excessive body fat in periods of abundance.

描述（由申请人提供）：肠道在脂质代谢和能量平衡中起着关键作用。它是膳食营养素的入口，含载脂蛋白B的乳糜微粒的来源，以及向其他组织发出当前营养状态信号以维持体内平衡和促进代谢效率的内分泌器官。然而，这些过程中涉及的许多分子机制仍然难以捉摸。我们研究的长期目标是了解肠道脂质加工如何调节全身脂质代谢和能量平衡。本提案的总体目标是阐明酰基辅酶A：单酰基甘油酰基转移酶-2（MGAT 2）调节全身能量平衡的机制。MGAT 2缺陷小鼠（Mgat 2-/-小鼠）显示出对由高脂肪喂养诱导的肥胖和相关代谢紊乱的显著抗性。与肠在调节食物摄入和营养同化中的公认作用相反，Mgat 2-/-小鼠的表型表明肠在调节能量消耗中的先前未被认识的作用。在已知的MGAT酶中，MGAT 2在人和小鼠的肠中高度表达。MGAT活性因其在膳食脂肪吸收中的作用而闻名，因为它催化三酰甘油的再合成，这是乳糜微粒形成的必要步骤，乳糜微粒将膳食脂肪输送到外周组织。尽管消耗和吸收了正常量的脂肪，Mgat 2-/-小鼠在高脂肪饮食中不会过度增重。然而，与对照组同窝出生的小鼠相比，这些小鼠消耗的能量明显更多。能量消耗的差异随着膳食脂肪的增加而增加。尽管Mgat 2-/-小鼠吸收正常量的脂肪，但更多的膳食脂肪是从远端肠道吸收的，从而延迟膳食脂肪进入循环。此外，他们的餐后葡萄糖依赖性促胰岛素肽（GIP）水平较低，而胰高血糖素样肽1（GLP 1）水平高于对照组。这两种肠道激素都会影响能量平衡。因此，我们假设MGAT 2协调肠细胞中乳糜微粒形成的脂质摄取和加工，并调节肠内分泌细胞分泌GIP和GLP 1。因此，肠道MGAT 2指导膳食脂肪的递送朝向储存，以有效地吸收这种热量密集的营养素。为了验证我们的假设，在目标1中，我们将阐明MGAT 2在肠道脂质代谢、乳糜微粒形成和肠道激素释放中的作用。在目标2中，我们将确定缺乏MGAT 2对膳食脂肪分布的功能后果。在目的3中，我们将使用仅在肠中表达MGAT 2的小鼠和以相同的精氨酸特异性方式缺乏MGAT 2的小鼠评估肠MGAT 2对全身能量平衡的总体影响。通过完成这些目标，我们将推进我们对肠道脂质代谢如何调节全身能量平衡的理解，这将是重要的，因为它将为理解脂肪同化建立一个新的范式，这是一个基本的生理过程，对于在不景气时期的生存至关重要，但可能导致过量的身体脂肪。