ShRNAmir-RV Library Storage and Dissemination

ShRNAmir-RV 文库存储和传播

• 批准号: 8711629
• 负责人: Sonia Sharma
• 金额: $ 3.77万
• 依托单位: LA JOLLA INSTITUTE FOR IMMUNOLOGY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8711629
• 关键词: Antiviral Agents; Cataloging; Catalogs; Databases; Genetic Screening; Glycerol; Goals; Immunity; Individual; Libraries; RNA Interference; Research Infrastructure; Role; T-Lymphocyte; Technology; United States National Institutes of Health; open source

Core C will store the libraries, and any individual constructs built for this consortium, as bacterial glycerol stocks. Furthermore, the goal of our consortium is to make this technology as "open source" as possible, and therefore this Core will handle all external requests for shRNAmir libraries or individual constructs. LIAI has an RNAi Center with an excellent infrastructure already in place for large scale library handling, storing, and disseminating. Core C has 3 roles: 1. Storage of the thousands of shRNAmir constructs. 2. Dissemination of libraries through the U19 groups, as bacterial clone replicates. 3. Dissemination of libraries and constructs throughout the NIH IMVC.

核心C将以细菌甘油的形式存储库以及为该聚生体构建的任何单个构建体 名的股票明细金额此外，我们联盟的目标是使这项技术尽可能“开源”， 因此，此核心将处理所有对shRNAmir库或单个构建体的外部请求。LIAI有 一个RNAi中心，拥有良好的基础设施，可用于大规模文库的处理、存储和 传播。 Core C有三个角色： 1.存储数千个shRNAmir构建体。 2.随着细菌克隆复制，通过U19群传播文库。 3.在NIH IMVC中传播文库和构建体。